快速检测抗斯氏狸殖吸虫抗体的金标免疫渗滤法的建立和应用

目的 :建立一种检测斯氏狸殖吸虫病患者血清抗体的快速、敏感和特异的新方法。方法 :以斯氏狸殖吸虫成虫可溶性抗原为上样抗原 ,胶体金标记羊抗人IgG为显色剂 ,建立检测斯氏狸殖吸虫病患者血清抗体的斑点金免疫渗滤法 (DIGFA) ;并以ELISA作平行对照。结果 :DIGFA和ELISA分别检测 83例斯氏狸殖吸虫病患者血清抗体 ,其阳性率分别为 95 .2 % (79/ 83)和 91 .6 % (76 / 83) ,两法差异无显著性 (P >0 .0 5 )。DIGFA分别检测正常人血清32例、华支睾吸虫病患者血清 2 3例和日本血吸虫病患者血清 36例 ,前者均为阴性 ,后两者的交叉反应率分别为 4 .3% (1 / 2 3)和 8.3% (3/ 36 ) ;DIGFA和ELISA两法的总符合率达 92 .3% (36 / 39)。结论 :DIGFA的特异性和敏感性与ELISA相近 ,但方法快速、试剂稳定 ,且不需特殊设备 ,适用于各级医疗单位和血清流行病学调查

ESTABLISHMENT AND APPLICATION OF DOT IMMUNOGOLD FILTRATION ASSAY IN ANTIBODY DETECTION FOR PAGUMOGONIMIASIS SKRJABINI

Objective: To establish a new, fast, sensitive and specific assay for detecting antibodies in pagumogonimiasis skrjabini. Method: Using pagumogonimus skrjabini adult worm antigen and IgG Labelled with colloidal gold as color developing agent, a dot immunogold filtration assay(DIGFA) for detecting pagumogonimus skrjabini antibodies was tested. ELISA was used as the parallel control. Result: The positive rates of DIGFA and ELISA in testing the sera from 83 proved cases were 95.2%(79/83) and 91.6%(76/83),respectively, the difference between the two assays being insignificant(P>0.05). The negative rate of DIGFA in healthy people was 100%(32/32). The cross reaction rates in 36 schistosomiasis cases and 23 clonorchiasis cases were 8.3%(3/36) and 4.3%(1/23), respectively. Both coincidence rates in comparison of DIGFA with ELISA were 92.3%(36/39). Conclusion: DIGFA is as sensitive and specific as the ELISA, and thus has the advantages of simplicity and saving specific equipment. Therefore, this method is fitful to diagnosis of Pagumogonimiasis skrjabini as well as epidemiological investigation.