毛囊皮脂腺在脂质体鬼臼毒素透皮吸收中的作用

目的　探讨脂质体鬼臼毒素混悬液透皮的机制。方法　实验大鼠涂抹 0 .5%脂质体鬼臼毒素混悬液 ,分别在涂药 1、2、3、4、6、8、12、16、2 4及 48h时取皮肤标本 ,常规石蜡切片 ,采用激光共聚焦显微成像仪 (CLSM )扫描 ,观察毛囊皮脂腺部位和非毛囊、皮脂腺部位单位面积鬼臼毒素的荧光量。结果　毛囊皮脂腺部位单位面积的荧光值在涂药后 8h达到高峰 ,而非毛囊皮脂腺部位达到高峰的时间是涂药后 12h ,并且毛囊皮脂腺部位的荧光值在各个时间点上均高于非毛囊皮脂腺部位 ,两者相比差异有显著性 (P <0 .0 0 1)。鬼臼毒素 48h荧光值曲线下面积 (AUC) ,毛囊皮脂腺部位是非毛囊皮脂腺部位的 1.63倍。结论　毛囊皮脂腺部位可能在脂质体鬼臼毒素制剂透皮过程中起主要作用

The Effects of Hair Follicle and Sebaceous Gland in Transdermal Absorption of a Liposome-encapsulated Formulation of Podoohvllotoxin Susoension

ObjectiveTo study transdermal absorption of l iposome podophyllotoxin suspension. MethodsTest raes were applied 0.5% liposome podophylloto xin suspension,to obtain skin specimen in 1、2、3、4、6、8、12 、16、24 and 48 h after applying drug,and to operate paraffin section, to observe unit area flu orescent amount in sites of hair follicle and sebaceous gland and other places u sing confocal laser scanning microscope (CLSM). ResultsThe time of the highest fluorescent amount were 8 h in sites of hair follicle and sebaceous gland, than other sites were 12 h,and the unit area fluorescent amount in sites of hair follicle and sebaceous gland w ere significantly higher than that in other sites all the time (P<0.001). Th e AUC of fluorescent in sites of hair follicle and sebaceous gland is 1.63-fold than that of other sites.ConclusionSites of hair follic le and sebaceous gland possibly have principal role in transdermal absorption of liposome podophyllotoxin suspension.