| HPLC测定众生丸中三种苷类成分的含量 |
| |
| 引用本文： | 陈瑞云,温勇,王德杭,何纯静,陈建南,蒋东旭. HPLC测定众生丸中三种苷类成分的含量[J]. 中药新药与临床药理, 2010, 21(2) |
| |
| 作者姓名： | 陈瑞云 温勇 王德杭 何纯静 陈建南 蒋东旭 |
| |
| 作者单位： | 1. 广州中医药大学新药研究开发中心,广州,510006
2. 广州中医药大学新药研究开发中心,广州510006;东莞广州中医药大学中医药数理工程研究院,东莞广东523808 |
| |
| 摘 要： | 目的用HPLC法测定众生丸中三种苷类成份的含量。方法采用高效液相色谱法对众生丸中三种苷类进行含量测定,检测波长为230nm,色谱柱为Kromasil C18(4.6mm×250mm,5μm),甲醇-0.4%磷酸为流动相梯度洗脱,流速为1.0min/mL,柱温为35℃。结果芍药苷进样量在0.1542μg～2.8384μg范围内呈良好的线性关系,平均回收率为97.82%,RSD为0.99%(n=6);虎杖苷进样量在0.0976μg～1.5920μg范围内呈良好的线性关系,平均回收率为97.44%,RSD为1.31%(n=6);黄芩苷进样量在0.7692μg～11.5380μg范围内呈良好的线性关系,平均回收率为99.79%,RSD为1.75%(n=6)。结论该法简便准确,重现性好,专属性强,可作为该制剂的质量控制。
|
| 关 键 词： | 众生丸 芍药苷 虎杖苷 黄芩苷 |
Determination of Three Glycosides in Traditional Chinese Medicine Zhongsheng Wan by HPLC |
| |
| CHEN Ruiyun,WEN Yong,WANG Dehang,HE Chunjing,CHEN Jiannan,JIANG Dongxu. Determination of Three Glycosides in Traditional Chinese Medicine Zhongsheng Wan by HPLC[J]. Traditional Chinese Drug Research & Clinical Pharmacology, 2010, 21(2) |
| |
| Authors: | CHEN Ruiyun WEN Yong WANG Dehang HE Chunjing CHEN Jiannan JIANG Dongxu |
| |
| Affiliation: | CHEN Ruiyun1,WEN Yong1,WANG Dehang1,HE Chunjing1,CHEN Jiannan1,JIANG Dongxu1,2(1.Research & Development Center of New Drugs,Guangzhou University of Chinese Medicine,Guangzhou 510006,China,2.Dong-guan Mathematical Engineering Academy of Chinese Medicine,Dongguan 523808 Guargzhou,China) |
| |
| Abstract: | Objective To determine the three Glycosides in traditional Chinese medicine Zhongsheng Wan by HPLC. Methods The chromatographic conditions included Kromasil C_(18) column (250 mm×4.6 mm, 5 μm) and the mobile phase consisting of a mixture of methanol- 0.4 %phosphoric acid at a flow rate of 1.0 mL/min at 35 ℃. The detection wavelength was 230 nm. Results The linear range of paeoniflorin was from 0.154 2 μg to 2.838 4 μg, and the average recovery was 97.82 %(RSD=0.99 %, n=6) . Piceid showed good linearity in the range of 0.097 6～1.592 0 μg, and the average recovery was 97.44 %(RSD=1.31 %, n=6). Baicalin showed good linearity in the range of 0.769 2～ 11.538 0μg, and the average recovery was 99.79 %(RSD=1.75 %, n=6). Conclusion This method is simple and reproducible, and can serve as the standard for controlling the quality of Zhongsheng Wan. |
| |
| Keywords: | HPLC Zhongsheng Wan Paeoniflorin Piceid Baicalin HPLC |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
