千里光抗金黄色葡萄球菌作用机制的血清药理学研究

目的 应用血清药理学方法研究中药千里光抗金黄色葡萄球菌的作用及其机制.方法 从千里光中分离出黄酮类化合物,然后通过给小鼠灌胃制备千里光水浸液的含药血清和黄酮类化合物的含药血清.用扫描电镜观察各含药血清对金黄色葡萄球菌超微结构的影响,用同位素前体参入试验观察各含药血清对金黄色葡萄球菌生物合成的影响.同时设立生理盐水血清作为阴性对照.结果 与阴性对照血清相比,两种含药血清作用后的金黄色葡萄球菌,其形态和超微结构均发生明显的变化,菌体塌陷,细菌融合成团,呈现肿胀模糊等变化;其DNA,RNA 蛋白质和肽聚糖合成也均受到明显的抑制.结论 千里光的抗金黄色葡萄球菌作用机制可能是通过抑制细菌的DNA,RNA,蛋白质和肽聚糖的合成有关,其作用的有效成分可能是黄酮类化合物.

Study on Mechanism of Senecio scandens Buch-Ham against Staphylococcus aureus with Serum Pharmacology

Objective To explore the antibacterial mechanism of Senecio scandens Buch-Ham(SSB) on Staphylococcus aureus by serum pharmacological method.Methods Flavonoid compound was isolated from SSB,and then the serum contained water decoct agent of SSB or flavonoid compound were prepared with mice.By scanning electronic microscope (SEM),the effects of drug-containing serum on the ultrastructure of S.aureus were observed.By incorporation experiment of isotope-labeled precursor,the effects of drug containing serum on the biosynthesis of S.aureus were observed as well.Meanwhile,the serum contained normal saline was set as negative control.Results Compared with negative control serum,the morphology and ultrastructure of both drug containing serum on S.aureus showed obvious changes:the cell collapsed,melt together,blurred and swollen,ect.In addition,the synthesis of DNA,RNA,protein and peptidoglycan was inhibited.Conclusion The antibacterial mechanism of SSB on S.aureus may be involved with the inhibition of DNA,RNA,protein and peptidoglycan synthesis,its effective ingredients may be flavonoid compound.