| 三氧化二砷对人肝癌SMMC-7721细胞侵袭转移能力的影响 |
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| 引用本文： | 余桂芳,陈益民,严跃红,曾文铤,朱科伦.三氧化二砷对人肝癌SMMC-7721细胞侵袭转移能力的影响[J].现代肿瘤医学,2011,19(7):1282-1284. |
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| 作者姓名： | 余桂芳 陈益民 严跃红 曾文铤 朱科伦 |
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| 作者单位： | 1. 广州医学院港湾医院肝病区,广东,广州,510700
2. 广州医学院第一附属医院肝病研究室,广东,广州,510120 |
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| 基金项目： | 广州市中医药科研基金资助项目 |
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| 摘 要： | 目的:探讨三氧化二砷(As2O3)对人肝癌SMMC-7721细胞黏附、侵袭、转移能力的影响以及其机制。方法:采用MTT法观察人肝癌SMMC-7721细胞经As2O3作用前后对基底膜黏附能力的影响;Transwell膜侵袭系统观察SMMC-7721细胞经As2O3作用前后游走与穿透基底膜能力的改变。免疫细胞化学方法检测人肝癌细胞经As2O3作用前后CD44V6表达的改变。结果:As2O3能显著抑制人肝癌SMMC-7721细胞与m arigel的黏附、抑制细胞游走与穿透基底膜的作用(P<0.05),能抑制人肝癌细胞CD44v6的表达(P<0.05)。结论:As2O3具有抑制人肝癌细胞的侵袭、转移能力,其抑制作用可能与CD44v6的表达下调有关。
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| 关 键 词： | 肝癌 SMMC-7721细胞 三氧化二砷 黏附 侵袭 CD44拼接异构体6 |
Effect of Arsenic trioxide on invasion and metastasis of human hepatocarcinoma cells SMMC-7721 in vitro |
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| YU Gui-fang,CHEN Yi-min,YAN Yue-hong,ZENG Wen-ting,ZHU Ke-lun.Effect of Arsenic trioxide on invasion and metastasis of human hepatocarcinoma cells SMMC-7721 in vitro[J].Journal of Modern Oncology,2011,19(7):1282-1284. |
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| Authors: | YU Gui-fang CHEN Yi-min YAN Yue-hong ZENG Wen-ting ZHU Ke-lun |
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| Institution: | YUGui-fang1,CHEN Yi-min1,YAN Yue-hong1,ZENG Wen-ting2,ZHUKe-lun21 Department of Hepatology,the Gangwan Hospital of Guangzhou Medical College,Guangzhou 510700,China,2Institute of Hepatology,the First Hospital,Guangzhou Medical College,Guangzhou 510120,China. |
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| Abstract: | Objective:To study the effect of Arsenic trioxide(As2O3) on adhesion and invasion metastasis of SMMC-7721 human liver cancer cell in vitro.Methods:MTTwas used to study the effect of adhesion by As2O3 on SMMC-7721 cell.The Transwell Chamber membrane invasion culture system was used to study the invasion and metastasis.The protein of CD44v6 was determined by immunocytochemical(ICC) staining.Results:As2O3 could significantly inhibit SMMC-7721cell adhesion with matrigel,movement and migration through matrigel t... |
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| Keywords: | liver cancer SMMC-7721 cell Arsenic trioxide adhesion invasion CD44v6 |
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