灯盏花素对脓毒症性急性肾损伤保护作用的实验研究

目的观察灯盏花素对脓毒血症模型大鼠急性肾损伤的保护作用。方法清洁级Wistar雄性大鼠60只,随机分为假手术组、脓毒血症模型组和灯盏花素大、中、小剂量组,每组12只。采用盲肠结扎穿孔术（CLP）复制大鼠脓毒血症模型。灯盏花大、中、小剂量组在造模后立即分别给予灯盏花素5、10、20mg/kg尾静脉注射。术后24h处死大鼠,下腔静脉取血测定肌酐（Scr）、血尿素氮（BUN）水平,采用酶联免疫法（ELISA）测定血清中性粒细胞明胶酶相关脂质运载蛋白（NGAL）、肾损伤分子1（KIM-1）的水平;摘取肾组织标本进行病理组织学观察,免疫印迹法检测肾脏NGAL和KIM-1蛋白含量。结果与脓毒血症组比较,灯盏花素大、中剂量组大鼠血清Scr、BUN、NGAL和KIM-1水平及肾组织NGAL和KIM-1蛋白表达水平均显著降低（P均〈0.05）;肾脏病理改变好转。灯盏花素小剂量组肾脏病理改变未见好转（P〉0.05）。结论灯盏花素可以显著改善脓毒血症模型大鼠肾组织损伤,降低血清NGAL和KIM-1水平及肾组织NGAL和KIM-1蛋白表达量,对脓毒血症所致急性肾损伤具有保护作用。

Protective effects of breviscapus on acute kidney injury in rats with sepsis

Objective To investigate the effect of breviscapine on acute kidney injury in a rat model of sepsis. Methods Sixty male Wistar rats were randomly （random number） assigned to sham operation group（n=12）, sepsis group（n=12）, and sepsis treated with breviscapine groups（36 rats were divided into 3 groups according to the dose, n=12）. The rat model of sepsis was established by cecal ligation and puncture （CLP）. The rats were injected once a day with breviseapine i.v.（5mg/kg, 10mg/kg, and 20mg/kg） after CLP were done; then the rats were killed after operations for 24 h to get blood and kidney. The levels of blood urea nitrogen （BUN） and serum creatinine（Scr） were assayed by enzymatic assays, the serum level of neutrophil gelatinase associate fipoprotein （NGAL） and kidney injury molecule 1 （KIM-1） was detected by ELISA, renal tissue injury was determined by HE, and the expression of NGAL and KIM-1 in kidney was detected by western blot. Results Compared with sepsis model, rats in breviscapine groups （10mg/kg and 20mg/kg） had the levels of BUN, Scr in plasma and NGAL and KIM-1 in both plasma and renal tissue decreased（all P〈0.05）, and improved renal pathology, but no significant improvement of renal pathology was observed in 5mg/kg breviscapine group （P〉0.05）. Conclusion Breviscapine can improve renal injury by reducing the level of NGAL and KIM-1 in plasma and renal tissue, thus it has protective effects on renal function in rats with sepsis.