| 表达大鼠IL-2、B7-1基因CBRH7919细胞的建立 |
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| 引用本文： | 史瑶平,郭燕,李鹤平,沈俊杰,杨建勇.表达大鼠IL-2、B7-1基因CBRH7919细胞的建立[J].世界华人消化杂志,2011(25):2660-2663. |
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| 作者姓名： | 史瑶平 郭燕 李鹤平 沈俊杰 杨建勇 |
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| 作者单位： | 中山大学附属第一医院放射科; |
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| 基金项目： | 国家自然科学基金面上资助项目,No.30600156,No.81071247,No.30870692~~ |
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| 摘 要： | 目的:研究携带大鼠IL-2、B7-1目的基因的CBRH7919细胞(即CBRH7919/IL-2/B7-1)体外表达目的基因的能力,方法:RT-PCR扩增Wistar大鼠IL-2、B7-1目的基因,分别克隆至重组逆转录病毒载体pBaBe-puro及pMSCV-neo,构建逆转录病毒载体pBaBe-puro-IL-2及p...
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| 关 键 词： | 白介素2 B7-1 CBRH7919 原发性肝癌 |
Establishment of a CBRH7919 cell line expressing rat IL-2 and B7-1 |
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| Yao-Ping Shi,Yan Guo,He-Ping Li,Jun-Jie Shen,Jian-Yong Yang.Establishment of a CBRH7919 cell line expressing rat IL-2 and B7-1[J].World Chinese Journal of Digestology,2011(25):2660-2663. |
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| Authors: | Yao-Ping Shi Yan Guo He-Ping Li Jun-Jie Shen Jian-Yong Yang |
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| Institution: | Yao-Ping Shi,Yan Guo,He-Ping Li,Jun-Jie Shen,Jian-Yong Yang,Department of Radiology,the First Affiliated Hospital of Sun Yat-sen University,Guangzhou 510080,Guangdong Province,China |
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| Abstract: | AIM: To establish a CBRH7919 cell line CBRH7919 expressing rat IL-2 and B7-1 genes and to examine their ability to express the IL-2 and B7-1 genes in vitro. METHODS: The IL-2 and B7-1 genes were amplified by RT-PCR and subcloned into retroviral vectors pBaBe-puro and pMSCV-neo, respectively, to obtain the recombinant retroviral vectors pBaBe-puro-IL-2 and pMSCV-neo-B7-1. The recombinant plasmids were then transfected into the 293FT packaging cells. The obtained infectious viruses were used to infect the CBR... |
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| Keywords: | Interleukin-2 B7-1 CBRH7919 Primary liver cancer |
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