| 沉默CXCR4基因对食管鳞癌EC-9706细胞中MMP-9基因表达的影响 |
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| 引用本文： | 刘剑,王峰,朱利楠,何炜,王留兴,樊青霞. 沉默CXCR4基因对食管鳞癌EC-9706细胞中MMP-9基因表达的影响[J]. 世界华人消化杂志, 2011, 0(28) |
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| 作者姓名： | 刘剑 王峰 朱利楠 何炜 王留兴 樊青霞 |
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| 作者单位： | 郑州大学第一附属医院肿瘤科; |
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| 基金项目： | 河南省科技厅科技攻关基金资助项目,No.72102310054~~ |
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| 摘 要： | 目的:探讨在食管鳞癌EC-9706细胞中沉默CXCR4基因对MMP-9基因表达的影响,为阐明CXCR4基因在食管鳞癌侵袭转移中的作用提供实验依据.方法:化学合成2条靶向CXCR4基因的siRNA1和siRNA2,同时设立荧光标记阴性对照和空白对照.脂质体法转染入EC-9706细胞,荧光显微镜下观察转染效率.转染48h后,半定量RT-PCR检测各组细胞CXCR4和MMP-9基因mRNA表达的变化,Western blot检测各组细胞CXCR4和MMP-9基因蛋白表达的变化,侵袭小室检测各组细胞穿膜细胞数的变化,MTT检测各组细胞的A值.结果:与阴性对照和空白对照相比,转染CXCR4siRNA1和siRNA2组细胞CXCR4mRNA和蛋白的表达明显降低,差异具有统计学意义(P<0.05);同时与阴性对照和空白对照相比,转染CXCR4siRNA1和siRNA2组细胞MMP-9mRNA和蛋白的表达同样明显降低,差异具有统计学意义(P<0.05);转染CXCR4siRNA1和siRNA2组细胞穿膜细胞数与阴性对照和空白对照相比明显下降,差异具有统计学意义(P<0.05);MTT结果显示转染CXCR4siRNA1和siR...
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| 关 键 词： | 食管鳞癌 CXC趋化因子受体4 基质金属蛋白酶-9 基因调控 |
SiRNA-mediated silencing of the CXCR4 gene downregulates MMP-9 expression in EC-9706 cells |
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| Jian Liu,Feng Wang,Li-Nan Zhu,Wei He,Liu-Xing Wang,Qing-Xia Fan. SiRNA-mediated silencing of the CXCR4 gene downregulates MMP-9 expression in EC-9706 cells[J]. World Chinese Journal of Digestology, 2011, 0(28) |
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| Authors: | Jian Liu Feng Wang Li-Nan Zhu Wei He Liu-Xing Wang Qing-Xia Fan |
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| Affiliation: | Jian Liu,Feng Wang,Li-Nan Zhu,Wei He,Liu-Xing Wang,Qing-Xia Fan,Department of Oncology,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,Henan Province,China |
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| Abstract: | AIM: To investigate the effect of small interfering RNA (siRNA)-mediated blockade of CXCR4 signaling on the expression of MMP-9 in human esophageal carcinoma cell line EC9706 and to provide an experimental foundation for further elucidating the role of CXCR4 in metastasis and invasion of esophageal carcinoma (ESCC). METHODS: Two siRNAs targeting the CXCR4 gene and one fluorescence-labeled negative con-trol siRNA were chemically synthesized and transfected into EC9706 cells, Non-transfected EC9706 cells were... |
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| Keywords: | Esophageal carcinoma CXC chemokine receptor 4 Matrix metalloproteinase-9 Gene regu-lation |
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