| CD117阳性细胞亚群在肝癌细胞系HepG2中的分离及其特性 |
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| 引用本文： | 容雁,王云霞,孙立新,张晓艳,杨治华,冉宇靓,孙力超. CD117阳性细胞亚群在肝癌细胞系HepG2中的分离及其特性[J]. 世界华人消化杂志, 2011, 0(36) |
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| 作者姓名： | 容雁 王云霞 孙立新 张晓艳 杨治华 冉宇靓 孙力超 |
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| 作者单位： | 广西医科大学附属肿瘤医院;中国医学科学院北京协和医学院肿瘤研究所分子肿瘤学国家重点实验室; |
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| 基金项目： | 国家重点基础研究发展计划(973计划)基金资助项目,No.2009CB521804; 国家科技重大专项基金资助项目,No.2011ZX09102-010-02~~ |
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| 摘 要： | 目的:观察从人肝癌细胞系HepG2中分离的CD117+细胞生物学行为,探讨肝癌中CD117+细胞亚群的干细胞特性.方法:采用无血清悬浮培养法培养HepG2细胞,流式细胞术检测HepG2及球体细胞中CD117的表达比例.用流式细胞分选技术从HepG2成球细胞中分离CD117+的肿瘤细胞,进行无血清悬浮培养,观察其成球能力.CCK-8法观察CD117+细胞的增殖能力和顺铂对CD117+细胞的抑制率,计算IC50和耐药指数(RI).结果:HepG2细胞能在无血清培养基中存活、增殖并形成细胞球,成球率为6.21%±2.03%;流式细胞检测发现球体细胞中CD117+细胞的比例比HepG2细胞提升了9倍;CD117+细胞在无血清培养基中成球率和增殖能力均显著高于未分选细胞和CD117细胞;CD117+细胞在各浓度的顺铂作用下抑制率均较未分选细胞和CD117细胞明显降低,三者IC50分别为12.229μmol/L、7.970μmol/L和7.345μmol/L,CD117+细胞和未分选细胞耐药系数RI为1.165和1.076.结论:人肝癌细胞系HepG2中的CD117+细胞是具有肿瘤干细胞特性的细胞亚群,CD117可能是肝...
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| 关 键 词： | 肝癌 肿瘤干细胞 HepG2 CD117 |
CD117-positive cells in human hepatocarcinoma cell line HepG2:isolation and evaluation of their stem cell properties |
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| Yan Rong,Xiao-Yan Zhang,Zhi-Hua Yang,Li-Chao Sun,the Affiliated Tumor Hospital of Guangxi Medical University,Nanning ,Guangxi Zhuang Autonomous Region,China Yun-Xia Wang,Li-Xin Sun,Yu-Liang Ran,State Key Laboratory of Molecular Oncology,Cancer Institute,Chinese Academy of Medical Sciences,Peking Union Medical College,Beijing ,China. CD117-positive cells in human hepatocarcinoma cell line HepG2:isolation and evaluation of their stem cell properties[J]. World Chinese Journal of Digestology, 2011, 0(36) |
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| Authors: | Yan Rong Xiao-Yan Zhang Zhi-Hua Yang Li-Chao Sun the Affiliated Tumor Hospital of Guangxi Medical University Nanning Guangxi Zhuang Autonomous Region China Yun-Xia Wang Li-Xin Sun Yu-Liang Ran State Key Laboratory of Molecular Oncology Cancer Institute Chinese Academy of Medical Sciences Peking Union Medical College Beijing China |
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| Affiliation: | Yan Rong,Xiao-Yan Zhang,Zhi-Hua Yang,Li-Chao Sun,the Affiliated Tumor Hospital of Guangxi Medical University,Nanning 530021,Guangxi Zhuang Autonomous Region,China Yun-Xia Wang,Li-Xin Sun,Yu-Liang Ran,State Key Laboratory of Molecular Oncology,Cancer Institute,Chinese Academy of Medical Sciences,Peking Union Medical College,Beijing 100021,China |
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| Abstract: | AIM:To isolate CD117+ cells from human hepatocarcinoma cell line HepG2 and to observe their biological behavior and stem cell properties.METHODS:HepG2 cells were cultured in serum-free medium.Flow cytometry was used to detect the expression of CD117 in HepG2 cells and sphere cells.CD117+ and CD117 cells were sorted by FACS and their ability of proliferation and self-renewal was observed in vitro.Chemosensitivity to cisplatin was determined by CCK-8 assay.The inhibitory rate of cells treated with cisplatin,t... |
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| Keywords: | Hepatocarcinoma Cancer stem cells HepG2 CD117 |
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