| 反义锁核酸与拉米夫定在HepG2.2.15细胞中抗HBV作用的比较 |
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| 引用本文： | 朱晓莹,王燕菲. 反义锁核酸与拉米夫定在HepG2.2.15细胞中抗HBV作用的比较[J]. 世界华人消化杂志, 2011, 0(28) |
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| 作者姓名： | 朱晓莹 王燕菲 |
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| 作者单位： | 右江民族医学院生物化学与分子生物学教研室;广州医学院生物化学与分子生物学教研室; |
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| 基金项目： | 广州市科技攻关计划基金资助项目,No.2002Z3-E4081~~ |
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| 摘 要： | 目的:应用反义锁核酸与拉米夫定作用HepG2.2.15细胞,对他们抗乙型肝炎病毒(hepatitis B virus,HBV)效果进行比较.方法:设计针对HBV翻译起始区S基因mRNA的反义寡核苷酸,并进行锁核酸修饰,以阳离子脂质体介导反义锁核酸转染HepG2.2.15细胞;拉米夫定组直接作用HepG2.2.15细胞;分别于用药后第2、4、6、8、10天收集细胞培养上清液.用ELISA法和FQ-PCR法检测收集上清液HBsAg、HBeAg和HBVDNA的含量.MTT法分别检测反义锁核酸与拉米夫定对细胞存活率的影响.结果:拉米夫定对HBVDNA具有明显抑制作用,最高可达46.52%,但对HBsAg、HBeAg影响较小;反义锁核酸对HBsAg、HBeAg及HBVDNA均有较强抑制作用,对HBsAg、HBeAg和HBVDNA的最高抑制率分别达67.69%、59.71%和62.96%(P<0.05),且抑制随时间呈增高趋势.反义锁核酸与拉米夫定对细胞代谢均无明显影响.结论:反义锁核酸抗HBV作用机制与拉米夫定不同,反义锁核酸抗HBV作用明显优于拉米夫定.
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| 关 键 词： | 乙型肝炎病毒 反义锁核酸 拉米夫定 HepG2.2.15细胞 抗病毒 |
Inhibitory effect of locked nucleic acid antisense oligonucleotides versus lamivudine on HBV replication in HepG2.2.15 cells |
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| Xiao-Ying Zhu. Inhibitory effect of locked nucleic acid antisense oligonucleotides versus lamivudine on HBV replication in HepG2.2.15 cells[J]. World Chinese Journal of Digestology, 2011, 0(28) |
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| Authors: | Xiao-Ying Zhu |
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| Affiliation: | Xiao-Ying Zhu,Department of Biochemistry and Molecular Biology,Youjiang Medical University for Nationalities,Baise 533000,Guangxi Zhuang Autonomous Region,ChinaYan-Fei Wang,Department of Biochemistry and MolecularBiology,Guangzhou Medical College,Guangzhou 510182,Guangdong Province,China |
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| Abstract: | AIM:To compare the inhibitory effect of locked nucleic acid antisense oligonucleotides(antisense-LNA) and lamivudine on HBV replication in HepG2.2.15 cells.METHODS:Antisense-LNA was introduced into HepG2.2.15 cells by cationic liposome-mediated transfection.Supernatants were collected 2,4,6,8,10 days after medication.The concentrations of HBsAg and HBeAg in cell supernatants were tested by ELISA.HBV DNA levels in cellsupernatants were determined by FQ-PCR.Cell toxicity of antisense-LNA and lamivudine was de... |
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| Keywords: | Hepatitis B virus Antisense LNA Lamivudine HepG2.2.15 cells Antiviral activity |
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