| 真核绿色荧光蛋白表达载体pEGFP-C1/PAK-1的构建及其在结直肠癌SW480细胞内的表达 |
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| 引用本文： | 武金宝,党彤,陈学清,张振书,张宏权,宋于刚.真核绿色荧光蛋白表达载体pEGFP-C1/PAK-1的构建及其在结直肠癌SW480细胞内的表达[J].世界华人消化杂志,2011(26). |
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| 作者姓名： | 武金宝 党彤 陈学清 张振书 张宏权 宋于刚 |
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| 作者单位： | 包头医学院第二附属医院内蒙古消化病研究所;广州医学院第一附属医院消化内科;南方医科大学南方医院消化研究所;Karolinska Institute Department of Biosciences and Nutrition;Sweden Huddinge SE-14157;北京大学基础医学院分子细胞生物学与肿瘤生物学研究室; |
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| 基金项目： | 国家自然科学基金资助项目,No.30570839; 内蒙古自治区自然科学基金资助项目,No.2010MS1101; 内蒙古自治区高等学校科学研究基金资助项目,No.NJ10183; 包头市社会发展科技支撑基金资助项目,No.2008R2001-1~~ |
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| 摘 要： | 目的:构建重组p21-activated kinase-1(PAK1)基因绿色荧光蛋白表达载体pEGFP-C1/PAK1,并转染入结直肠癌细胞SW480中表达.方法:在南方医科大学附属南方医院消化研究所实验室,从人类结直肠癌细胞株SW620细胞提取总RNA,经逆转录聚合酶链式反应获得人PAK1 cDNA片段,经过限制性内切酶进行酶切,T4连接酶进行连接,将目的基因克隆至真核绿色荧光蛋白表达载体pEGFP-C1上,然后转染结直肠癌细胞株SW480,观察其在细胞中表达.结果:重组载体经限制性内切酶酶切鉴定和DNA序列分析验证,显示插入载体的序列与目的基因一致,而且该重组载体能够在SW480细胞中表达.结论:成功构建了真核绿色荧光蛋白表达载体pEGFP-C1/PAK1,为研究PAK1在结直肠癌中的生物学功能奠定了基础.
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| 关 键 词： | p21-activated kinase-1 结直肠癌 真核表达 绿色荧光蛋白 基因克隆 |
Construction of a eukaryotic green fluorescent protein expression vector carrying the PAK-1 gene(pEGFP-C1/PAK1) and its expression in SW480 cells |
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| Jin-Bao Wu,Tong Dang,Inner Mongolia Institute of Digestive Diseases,the Second Affiliated Hospital of Baotou Medical College,Baotou ,Inner Mongolian Autonomous Region,China Xue-Qing Chen.Construction of a eukaryotic green fluorescent protein expression vector carrying the PAK-1 gene(pEGFP-C1/PAK1) and its expression in SW480 cells[J].World Chinese Journal of Digestology,2011(26). |
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| Authors: | Jin-Bao Wu Tong Dang Inner Mongolia Institute of Digestive Diseases the Second Affiliated Hospital of Baotou Medical College Baotou Inner Mongolian Autonomous Region China Xue-Qing Chen |
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| Institution: | Jin-Bao Wu,Tong Dang,Inner Mongolia Institute of Digestive Diseases,the Second Affiliated Hospital of Baotou Medical College,Baotou 014030,Inner Mongolian Autonomous Region,China Xue-Qing Chen,Department of Gastroenterology,the First Affiliated Hospital of Guangzhou Medical College,Guangzhou 510120,Guangdong Province,China Zhen-Shu Zhang,Yu-Gang Song,Institute of Digestive Diseases,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China Hong-Quan Zhang,Department of Bioscience... |
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| Abstract: | |
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| Keywords: | p21-activated kinase-1 Colorectal carcinoma Eukaryotic expression Green fluorescent protein Gene cloning |
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