| RNAi特异性抑制FoxO3a对软脂酸诱导HepG2.2.15细胞凋亡的影响 |
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| 引用本文： | 熊清芳,谢玉桃.RNAi特异性抑制FoxO3a对软脂酸诱导HepG2.2.15细胞凋亡的影响[J].世界华人消化杂志,2011(25):2623-2628. |
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| 作者姓名： | 熊清芳 谢玉桃 |
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| 作者单位： | 中南大学湘雅医院传染科; |
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| 基金项目： | 湖南省自然科学基金资助项目,No.09JJ5031~~ |
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| 摘 要： | 目的:观察FoxO3a基因干扰对软脂酸诱导HepG2.2.15细胞凋亡的影响.方法:HepG2.2.15细胞分五组:mock组(加脂质体)、FoxO3a siRNA组、FoxO3a siRNA+软脂酸组、阴性siRNA对照组、阴性siRNA+软脂酸组；Western blot法检测细胞的FoxO3a蛋白表达水平.MTT...
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| 关 键 词： | 小干扰RNA FoxO3a 软脂酸 凋亡 Bim p27Kip caspase-3 |
RNA interference-mediated silencing of the FoxO3a gene inhibits palmitate-induced apoptosis in human hepatoma cell line HepG2.2.15 |
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| Qing-Fang Xiong,Yu-Tao Xie.RNA interference-mediated silencing of the FoxO3a gene inhibits palmitate-induced apoptosis in human hepatoma cell line HepG2.2.15[J].World Chinese Journal of Digestology,2011(25):2623-2628. |
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| Authors: | Qing-Fang Xiong Yu-Tao Xie |
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| Institution: | Qing-Fang Xiong,Yu-Tao Xie,Department of Infectious Diseases,Xiangya Hospital,Central South University,Changsha 410008,China |
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| Abstract: | AIM: To investigate the impact of small interfering RNA (siRNA)-mediated silencing of the FoxO3a gene on palmitate-induced apoptosis of HepG2.2.15 cells. METHODS: Cultured HepG2.2.15 cells were divided into five groups: mock group (cells cultured in DMEM medium containing LipofectamineTM2000), FoxO3a siRNA group, FoxO3a siRNA+palmitate group, negative siRNA group, and negative siRNA+palmitate group. The protein expression of FoxO3a was detected by Western blot. Cell viability was measured by MTT assay. Apop... |
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| Keywords: | siRNA FoxO3a Palmitate Apoptosis Bim p27Kip Caspase-3 |
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