| IHC和FISH检测胃癌Her-2蛋白表达及基因扩增的比较 |
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| 引用本文： | 刘学政,陈铭,张家均,董虹,柳文菊. IHC和FISH检测胃癌Her-2蛋白表达及基因扩增的比较[J]. 世界华人消化杂志, 2011, 0(25) |
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| 作者姓名： | 刘学政 陈铭 张家均 董虹 柳文菊 |
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| 作者单位： | 湖北省荆州市第一人民医院检验科; |
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| 摘 要： | 目的:对比研究胃癌组织中免疫组织化学法(IHC)检测Her-2蛋白表达和荧光原位杂交法(FISH)检测Her-2基因扩增结果的差异性和相关性,并评估其临床应用价值.方法:采用EliVisionTMplus免疫组织化学染色方法和荧光原位杂交法检测118例胃癌组织中Her-2蛋白表达及Her-2基因扩增状况,并进行对比分析.结果:118例胃癌组织中,IHC检测Her-2蛋白表达(0)40例,(+)33例,(++)16例,(+++)29例,Her-2蛋白过表达率24.6%;FISH检测Her-2基因扩增38例,无扩增80例,Her-2基因扩增率32.2%.40例Her-2蛋白表达为(0)的标本中38例经FISH检测Her-2基因无扩增(95.0%);33例表达为(+)的标本中30例无扩增(90.9%);16例表达为(++)的标本中9例无扩增,7例扩增;29例表达为(+++)的标本中26例扩增(89.7%).结论:Her-2蛋白表达为(0-+)或(+++)时,IHC和FISH检测结果较一致,两种方法总符合率为85.6%,两者呈显著相关(P<0.01),IHC可作为初步筛查胃癌Her-2的首选方法.当Her-2表达为(...
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| 关 键 词： | 胃癌 Her-2 免疫组织化学 荧光原位杂交 |
Fluorescence in situ hybridization versus immunohistochemistry in the detection of Her-2 gene amplification and protein expression in gastric cancer |
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| Abstract: | AIM: To compare the difference and correlation between immunohistochemistry (IHC) to detect Her-2 protein expression and fluorescent in situ hybridization (FISH) to detect Her-2 gene amplification in gastric cancer and to assess their clinical value. METHODS: Immunohistochemistry and FISH were used to detect Her-2 protein expression and Her-2 gene amplification in 118 cases of gastric carcinoma, respectively. A comparative analysis was then performed. RESULTS: Of 118 cases of gastric carcinoma, 40 showed no... |
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| Keywords: | Gastric cancer Her-2 Immunohistochemistry Fluorescence in situ hybridization |
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