| mTOR与JNK信号通路在人结肠癌HT-29细胞中的作用及相互关系 |
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| 引用本文： | 张志永,张谢夫.mTOR与JNK信号通路在人结肠癌HT-29细胞中的作用及相互关系[J].世界华人消化杂志,2011(26). |
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| 作者姓名： | 张志永 张谢夫 |
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| 作者单位： | 郑州大学第一附属医院普通外科; |
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| 摘 要： | 目的:探讨mTOR和JNK信号通路在结肠癌中作用及可能的相互作用关系.方法:免疫方法检测p-mTOR及p-JNK在结肠癌组织和正常结肠组织中的表达情况.体外培养人结肠癌细胞株HT-29细胞,转染mTOR siRNA抑制mTOR表达,使用JNK抑制剂SP600125抑制JNK表达.Western blot法分别检测抑制mTOR和JNK后HT-29细胞中p-JNK、p-mTOR蛋白的表达.结果:在人结肠癌组织中p-mTOR和p-JNK表达阳性率分别为60%和56%,且两者阳性表达相关性分析存在正相关性(r=0.480,P<0.01).HT-29细胞株中抑制mTOR信号通路后mTOR siRNA转染组和空白对照组、Control siRNA组比较增殖(A值)明显降低,差异具有统计学意义(0.275±0.033,0.460±0.376vs0.479±0.012,均P<0.01),mTOR siRNA转染组凋亡指数明显高于空白对照组和Control siRNA组,差异具有统计学意义(12.330±1.533,1.000±0.147vs1.667±0.577,均P<0.01).抑制JNK信号通路后可见随着SP600125...
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| 关 键 词： | 结肠癌 哺乳动物雷帕霉素靶蛋白 c-Jun氨基末端酶 |
Interaction and relationship between mTOR and JNK signal pathways in human colon cancer cell line HT-29 |
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| Zhi-Yong Zhang,Xie-Fu Zhang.Interaction and relationship between mTOR and JNK signal pathways in human colon cancer cell line HT-29[J].World Chinese Journal of Digestology,2011(26). |
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| Authors: | Zhi-Yong Zhang Xie-Fu Zhang |
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| Institution: | Zhi-Yong Zhang,Xie-Fu Zhang,Department of General Surgery,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,Henan Province,China |
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| Abstract: | AIM:To investigate the possible interaction and relationship between mammalian target of rapamycin(mTOR) and c-Jun N-terminal kinase(JNK) signal pathways in colon cancer.MEHTODS:Immunohistochemical methods were applied to detect p-JNK and p-mTOR expression in colon cancer tissues.Human colon cancer cell line HT29 was cultured in vitro.The expression of mTOR protein was inhibited using specific siRNA,and JNK protein was inhibited with JNK inhibiter SP600125.The expression of p-JNK and p-mTOR proteins was det... |
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| Keywords: | Colon cancer Mammalian target of rapamycin C-Jun N-terminal kinase |
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