| 结合基因芯片表达谱研究miRNAs在食管鳞癌中的作用 |
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| 引用本文： | 吴耀松,陈玉龙,尹素改,周发祥. 结合基因芯片表达谱研究miRNAs在食管鳞癌中的作用[J]. 世界华人消化杂志, 2011, 0(36) |
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| 作者姓名： | 吴耀松 陈玉龙 尹素改 周发祥 |
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| 作者单位： | 河南中医学院; |
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| 基金项目： | 国家自然科学基金资助项目,No.81173177; 河南省教育厅自然科学基金资助项目,No.2010B360004; 河南中医学院科技创新人才基金资助项目,No.2010XCXR C02~~ |
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| 摘 要： | 目的:探讨食管鳞状细胞癌(esophageal squamous cell cancer,ESCC)中miRNAs的作用.方法:利用基因芯片来分析ESCC和正常组织间的miRNAs和mRNA的表达差异;为了研究差异miRNA功能,参考基因mRNA表达数据来分析miRNA靶基因和调节基因.结果:得到了8个差异的miRNA和1178个差异的mRNA.8个miRNA具有142个靶基因,137个基因被miRNA具有转录因子活性的靶基因调节.其中,53个基因参与KEGG信号通路,14个基因与BioCarta通路相关,137个基因发生相互作用,构成433边的相互作用网络.结论:miRNA的调节功能异常可能是导致ESCC基因表达失常的原因,8个miRNA和受他们调节的基因组成一个相互调节网络,并在ESCC中发挥重要作用.
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| 关 键 词： | 食管鳞癌 芯片 miRNA |
Identification of differentially expressed miRNAs and mRNAs in esophageal squamous cell cancer by gene microarray |
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| Yao-Song Wu,Yu-Long Chen,Su-Gai Yin,Fa-Xiang Zhou,Laboratory of Molecular Biology,Henan University of Traditional Chinese Medicine,Zhengzhou ,Henan Province,China. Identification of differentially expressed miRNAs and mRNAs in esophageal squamous cell cancer by gene microarray[J]. World Chinese Journal of Digestology, 2011, 0(36) |
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| Authors: | Yao-Song Wu Yu-Long Chen Su-Gai Yin Fa-Xiang Zhou Laboratory of Molecular Biology Henan University of Traditional Chinese Medicine Zhengzhou Henan Province China |
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| Affiliation: | Yao-Song Wu,Yu-Long Chen,Su-Gai Yin,Fa-Xiang Zhou,Laboratory of Molecular Biology,Henan University of Traditional Chinese Medicine,Zhengzhou 450008,Henan Province,China |
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| Abstract: | AIM:To identify differentially expressed microRNAs (miRNAs) and mRNAs in esophageal squamous cell cancer (ESCC).METHODS:Differentially expressed miRNAs and mRNAs between ESCC and normal esophageal mucosal tissue were identified by gene microarray.To study the functions of differentially expressed miRNAs,their target genes and genes regulated by these target genes were analyzed using online bioinformatic tools.RESULTS:Eight differentially expressed miR-NAs and 1 178 mRNAs were identified.Eight miRNAs had 142... |
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| Keywords: | Esophageal squamous cell cancer Microarray MicroRNAs |
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