| siRNA沉默Bmi-1基因对人肝癌细胞株MHCC97-H侵袭迁移能力的影响 |
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| 引用本文： | 李小磊,宋文杰,陈战,陈亚峰,李霄,周亮,张福琴,窦科峰.siRNA沉默Bmi-1基因对人肝癌细胞株MHCC97-H侵袭迁移能力的影响[J].世界华人消化杂志,2011(36). |
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| 作者姓名： | 李小磊 宋文杰 陈战 陈亚峰 李霄 周亮 张福琴 窦科峰 |
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| 作者单位： | 中国人民解放军第四军医大学西京医院肝胆胰脾外科; |
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| 摘 要： | 目的:探讨RNA干扰技术沉默Bmi-1基因表达后,对人肝癌细胞株MHCC97-H侵袭迁移能力的影响.方法:设计并合成针对Bmi-1基因序列特异性的双链小干扰RNA(Bmi-1-siRNA),转染高转移性人肝癌细胞株MHCC97-H,用流式细胞仪观察转染效率,荧光实时定量PCR和Westernblot检测Bmi-1基因的mRNA和蛋白表达水平;通过体外Transwell小室基质侵袭和迁移实验,观察Bmi-1表达沉默后对人肝癌细胞株MHCC97-H侵袭和迁移能力的影响.结果:将针对Bmi-1基因序列特异性的小干扰RNA(Bmi-1-siRNA)转染高转移性人肝癌细胞株MHCC97-H后,流式细胞仪显示,转染效率可达到91%.与空白组、对照siRNA组相比,实验组Bmi-1-siRNA能有效抑制MHCC97-H细胞中Bmi-1基因的mRNA(F=56.199,P<0.05)和蛋白表达水平.通过Transwell小室基质侵袭和迁移实验,我们分析了不同组细胞的侵袭迁移能力.结果发现,与空白组、对照siRNA组相比,Bmi-1-siRNA转染的MHCC97-H细胞穿透能力明显降低(F=186.66,12.746,P<0....
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| 关 键 词： | RNA干扰 肝肿瘤 Bmi-1 侵袭 转移 |
SiRNA-mediated silencing of the Bmi-1gene inhibits cell invasion and metastasis in human hepatocellular carcinoma cell line MHCC97-H |
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| Xiao-Lei Li,Wen-Jie Song,Zhan Chen,Ya-Feng Chen,Xiao Li,Liang Zhou,Fu-Qin Zhang,Ke-Feng Dou.SiRNA-mediated silencing of the Bmi-1gene inhibits cell invasion and metastasis in human hepatocellular carcinoma cell line MHCC97-H[J].World Chinese Journal of Digestology,2011(36). |
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| Authors: | Xiao-Lei Li Wen-Jie Song Zhan Chen Ya-Feng Chen Xiao Li Liang Zhou Fu-Qin Zhang Ke-Feng Dou |
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| Institution: | Xiao-Lei Li,Wen-Jie Song,Zhan Chen,Ya-Feng Chen,Xiao Li,Liang Zhou,Fu-Qin Zhang,Ke-Feng Dou,Department of Hepatobiliary,Pancreatic and Splenic Surgery,Xijing Hospital,The Fourth Military Medical University,Xi'an 710032,Shaanxi Province,China |
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| Abstract: | AIM:To investigate the effect of small interference RNA (siRNA)-mediated silencing of the Bmi-1gene on cell invasion and metastasis in human hepatocellular carcinoma cell line MHCC97-H.METHODS:Chemically synthesized siRNA duplex targeting the Bmi-1 gene (Bmi-1-siRNA) was transiently transfected into MHCC97-H cells,which have high metastatic potential,using Lipofectamine 2000.Transfection efficiency was evaluated by flow cytometry (FCM).Bmi-1 mRNA and protein expression was detected by real-time RT-PCR and W... |
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| Keywords: | RNA interference Liver neoplasms Bmi-1 Invasion Metastasis |
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