| 盐酸戊乙奎醚对大鼠急性坏死性胰腺炎肺损伤MIF和AQP-5表达的影响 |
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| 引用本文： | 蔡丹磊.盐酸戊乙奎醚对大鼠急性坏死性胰腺炎肺损伤MIF和AQP-5表达的影响[J].世界华人消化杂志,2011(36). |
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| 作者姓名： | 蔡丹磊 |
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| 作者单位： | 南通大学附属医院急诊内科; |
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| 摘 要： | 目的:探讨盐酸戊乙奎醚对大鼠急性坏死性胰腺炎(acute necrotic pancreatitis,ANP)并发急性肺损伤(acute lung injury,ALI)血清巨噬细胞移动抑制因子(macrophage migration inhibitory factor,MIF)和肺泡灌洗液水通道蛋白-5(aquaporin-5,AQP-5)表达的影响.方法:采用经胰胆管内逆行注射4%牛磺胆酸钠构建大鼠ANP并发ALI模型,100只SD大鼠以是否接受盐酸戊乙奎醚(penehyclidine hydrochloride,PHCD)腹腔注射干预治疗随机分为治疗组和非治疗组,每组再以6h、12h、24h、36h、48h五个取材时间点继续随机分组,共10组,每组10只动物.采用ELISA法测定血清中MIF的浓度和肺泡灌洗液中AQP-5的浓度,同时进行胰腺和肺组织病理形态学观察,肺组织湿/干重比检测,动脉血气分析.结果:相同时间点PHCD治疗组与非治疗组比较显示,血清MIF浓度降低(χ2=20.52,P=0.042),肺泡灌洗液AQ P-5浓度增高(χ2=17.22,P=0.044),胰腺和肺组织病理学损伤减轻,肺组...
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| 关 键 词： | 急性坏死性胰腺炎 急性肺损伤 盐酸戊乙奎醚 巨噬细胞移动抑制因子 水通道蛋白-5 |
Treatment with penehyclidine hydrochloride reduces the levels of MIF in serum and AQP-5 in bronchoalveolar lavage fluid in rats with acute necrotizing pancreatitis and acute lung injury |
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| Dan-Lei Cai,Emergency.Treatment with penehyclidine hydrochloride reduces the levels of MIF in serum and AQP-5 in bronchoalveolar lavage fluid in rats with acute necrotizing pancreatitis and acute lung injury[J].World Chinese Journal of Digestology,2011(36). |
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| Authors: | Dan-Lei Cai Emergency |
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| Institution: | Dan-Lei Cai,Emergency Department of Emergency Medicine,the Affiliated Hospital of Nantong University,Nantong 226001,Jiangsu Province,China |
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| Abstract: | AIM:To investigate the influence of treatment with penehyclidine hydrochloride (PHCD) on the levels of macrophage migration inhibitor factor (MIF) in serum and aquaporin-5 (AQP-5) in bronchoalveolar lavage fluid (BALF) in rats with acute necrotizing pancreatitis (ANP) and acute lung injury (ALI).METHODS:ANP and ALI was induced in Sprague-Dawley rats by retrograde injection of 4% sodium taurocholate into the biliopancreatic duct.One hundred rats were randomly and equally divided into treatment group and nont... |
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| Keywords: | Acute necrotizing pancreatitis Acute lung injury Penehyclidine hydrochloride Macrophage migration inhibitor factor Aquaporin-5 |
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