|
|||||
|
|
| 胶州湾具抗菌活性海洋链霉菌的筛选及菌株M097的代谢产物 | |
| 引用本文： | 崔洪霞,王淑军,李富超,秦松,Khaled Shaaban. 胶州湾具抗菌活性海洋链霉菌的筛选及菌株M097的代谢产物[J]. 中国天然药物, 2006, 4(4): 308-311 |
| 作者姓名： | 崔洪霞 王淑军 李富超 秦松 Khaled Shaaban |
| 作者单位： | 1. 中国科学院海洋研究所,青岛,266071;中国科学院研究生院,北京,100039 2. 淮海工学院,连云港,222005 3. 中国科学院海洋研究所,青岛,266071 4. 德国哥廷根大学有机生物分子化学系,哥廷根,D-37077 |
| 基金项目： | 山东省青岛市科技发展基金;江苏省重点建设实验室基金 |
| 摘 要： | 目的:筛选具有抗菌活性的海洋链霉菌并分离鉴定其代谢产物。方法:利用8株受试菌作为抗菌活性筛选模型,以杯碟法对采集自胶州湾海洋的256株链霉菌进行抗菌活性的筛选;对具有抗菌活性的海洋链霉菌M097进行发酵条件的优化,采用乙酸乙酯提取和柱层析等方法,分离发酵液中的代谢产物。结果:具有抗菌活性海洋链霉菌的比例为22%;通过ESI-MS、EI-MS、NMR等波谱数据进行分析,鉴定了3个活性代谢产物的结构。结论:具有抗菌活性的海洋链霉菌的比例很高;海洋链霉菌M097在M 2培养基中产生的3个活性代谢产物均属于大环内酯类抗生素,与Streptomyces griseus产生的feigrisolides A、B和C的结构一致。 |
| 关 键 词： | 海洋链霉菌 代谢产物 受试菌株 |
| 文章编号： | 1672-3651（2006）04-0308-04 |
| 收稿时间： | 2004-07-30 |
| 修稿时间： | 2006-06-10 |
Screening of Marine Streptomyces with Antibacterial Activity from JiaoZhou Bay and Studies on Metabolites from Strain M097 |
|
| CUI Hong-Xia,WANG Shu-Jun,LI Fu-Chao,QIN Song,Khaled Shaaban. Screening of Marine Streptomyces with Antibacterial Activity from JiaoZhou Bay and Studies on Metabolites from Strain M097[J]. Chinese JOurnal of Natural Medicines, 2006, 4(4): 308-311 | |
| Authors: | CUI Hong-Xia WANG Shu-Jun LI Fu-Chao QIN Song Khaled Shaaban |
| Affiliation: | 1.Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071 ; 2.Graduate School, Chinese Academy of Sciences, Beijing 100093, China 3.Department of Organic Chemistry, University of Goettingen, Goettingen, Germany D-37077 |
| Abstract: | AIM:To get inhibitory bacteria compounds from marine Streptomyces. METHOD:Using 8 test strains, 256 Streptomyces from ocean were screened with their activities against bacteria. Strain M097 was selected to get an optimum condition. Active compounds have been isolated from the crude extraction by fermentation, ethyl acetate extraction, column chromatography etc. RESULT:The proportion of active marine streptomyces was 22%. 3 compounds produced by marine Streptomyces M097 were identified as feigrisolides A, B and C respectively. CONCLUSION:Marine Streptomycetes showed promising prospects in discovery of inhibitory bacterial substances. |
| Keywords: | M097 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |