Human Cytomegalovirus Nuclear Egress Complex Subunit,UL53, Associates with Capsids and Myosin Va,but Is Not Important for Capsid Localization towards the Nuclear Periphery |
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Authors: | Adrian R. Wilkie Mayuri Sharma Margaret Coughlin Jean M. Pesola Maria Ericsson Jessica L. Lawler Rosio Fernandez Donald M. Coen |
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Affiliation: | 1.Department of Biological Chemistry and Molecular Pharmacology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USA; (A.R.W.); (M.S.); (J.M.P.); (J.L.L.); (R.F.);2.Committee on Virology, Harvard University, Cambridge, MA 02138, USA;3.Department of Systems Biology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USA;4.Electron Microscopy Core Facility, Department of Cell Biology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USA; |
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Abstract: | After herpesviruses encapsidate their genomes in replication compartments (RCs) within the nuclear interior, capsids migrate to the inner nuclear membrane (INM) for nuclear egress. For human cytomegalovirus (HCMV), capsid migration depends at least in part on nuclear myosin Va. It has been reported for certain herpesviruses that the nucleoplasmic subunit of the viral nuclear egress complex (NEC) is important for this migration. To address whether this is true for HCMV, we used mass spectrometry and multiple other methods to investigate associations among the HCMV NEC nucleoplasmic subunit, UL53, myosin Va, major capsid protein, and/or capsids. We also generated complementing cells to derive and test HCMV mutants null for UL53 or the INM NEC subunit, UL50, for their importance for these associations and, using electron microscopy, for intranuclear distribution of capsids. We found modest associations among the proteins tested, which were enhanced in the absence of UL50. However, we found no role for UL53 in the interactions of myosin Va with capsids or the percentage of capsids outside RC-like inclusions in the nucleus. Thus, UL53 associates somewhat with myosin Va and capsids, but, contrary to reports regarding its homologs in other herpesviruses, is not important for migration of capsids towards the INM. |
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Keywords: | capsid migration human cytomegalovirus UL53 UL50 myosin Va major capsid protein mass spectrometry virus genetics complementing cells null mutants |
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