异种脱蛋白BMP复合骨修复骨缺损实验研究

异种骨移植常由于强烈的免疫排斥反应失败。本文报告将小牛骨经脱蛋白处理（即脱去主要的抗原物质），后再复合进去骨形成蛋白（ｂｏｖｉｎｅＢｏｎｅＭｏｒｐｈｏｇｅｎｅｔｉｃｐｒｏｔｅｉｎ，简称ｂ－ＢＭＰ）使其成为既无抗原性，又利于骨形成的异种脱蛋白ＢＭＰ复合骨。将其植入新西兰白兔尺骨缺损（２ｃｍ），观察愈合结果。免疫学、放射学和组织学检查表明，植入异种脱蛋白ＢＭＰ复合骨，各组实验动物术后无任何免疫反应。４周均显示植入骨与骨床界线模糊，８周植入骨内可见大量成片新生骨细胞及新生血管长入。实验结果表明经处理后的大块异种骨移植，不但无任何免疫排斥反应，且可达到预期修复骨缺损的目的。

Experimental reseach on the restoration of bone defact with the complex of helorogenous deproteinized bone and the bone morphogenetic protein

The filure of the implantation of the heterogenous deproteinized bone is always due to the intensive immune rejection. A new method for treating heterogenous bone was discribed in this paper. The bone of calf was deproteinized, i. e. extracted the main antigens and combined with bovine bone morphogenetic protein(BMMP) and then produced a kind of heterogenous deproteinized bone, not only without antigenicity, but also advantageous to the bone formation. Implanting this kinds of bone comples into the artificial defact(2cm) of the radius of Newzeland rabbit, the observation on the recovery with immunological, radioactive, and histological methods, demonstrated that there was without any immune rejection in various kinds of experimental groups and there was indistinct margin between the implant and the fracture bed at 4th week; There were a great amount of lamella of new osteocytes and neogenetic vessels invension into the implant, at the 8th week.The experimental result indicates that the large heterogenous bone implantation, treaed beforehand, can not only without any immune rejection, but also can achieve the expected restoration.