| 两株鼠抗人VSIG4单克隆抗体的研制及其生物学特性的研究 |
| |
| 引用本文： | 张世杰,王蕾,汪家敏,陈曦,蒋靓,王月颖,胡玉敏,张学光,顾宗江. 两株鼠抗人VSIG4单克隆抗体的研制及其生物学特性的研究[J]. 中国免疫学杂志, 2010, 26(1) |
| |
| 作者姓名： | 张世杰 王蕾 汪家敏 陈曦 蒋靓 王月颖 胡玉敏 张学光 顾宗江 |
| |
| 作者单位： | 1. 苏州大学医学生物技术研究所,苏州,215007
2. 苏州大学附属第一医院心内科,苏州,215006 |
| |
| 摘 要： | 目的:制备鼠抗人vSIG4分子单克隆抗体(mAb)及对其生物学特性进行鉴定.方法:以高表达膜型VSIG4分子的基因转染细胞L929/VSIC4L为免疫原,常规免疫BALB/c小鼠,采用杂交瘤技术,经流式细胞术检测和多次;隆化培养,筛选出特异分泌鼠抗人VSIG4分子的杂交瘤细胞株;采用间接免疫荧光法、western blot、竞争抑制试验及MTT增殖试验等单对抗的生物学特征进行鉴定.结果:获得2株持续、稳定分泌鼠抗人VSIG4 mAb的杂交瘤细胞株,分别命名为9A7和9D5.对mAb生物学特性的研究结果表明,2株mAb均能识别巨噬细胞以及jurkat、THP-1、H446等肿瘤细胞株表面的VSIG4分子.对T细胞增殖抑制的阻断实验显示,这2株抗体对VSIG4分子抑制T细胞增殖均有阻断作用.结论:成功地获得了2株鼠抗人VSIG4功能性mAb,为进一步研究VSIG4及其未知受体的生物学功能奠定了基础.
|
| 关 键 词： | 杂交瘤 单克隆抗体 巨噬细胞 |
Preparation and characterization of two monoclonal antibodies against human VSIG4 |
| |
| ZHANG Shi-Jie,WANG Lei,WANG Jia-Min,CHEN Xi,JIANG Jing,WANG Yue-Ying,HU Yu-Min,ZHANG Xue-Guang,GU Zong-Jiang. Preparation and characterization of two monoclonal antibodies against human VSIG4[J]. Chinese Journal of Immunology, 2010, 26(1) |
| |
| Authors: | ZHANG Shi-Jie WANG Lei WANG Jia-Min CHEN Xi JIANG Jing WANG Yue-Ying HU Yu-Min ZHANG Xue-Guang GU Zong-Jiang |
| |
| Affiliation: | ZHANG Shi-Jie,WANG Lei,WANG Jia-Min,CHEN Xi,JIANG Jing,WANG Yue-Ying,HU Yu-Min,ZHANG Xue-Guang,GU Zong-Jiang.Biotechnology Institute of Soochow University,Suzhou 215007,China |
| |
| Abstract: | Objective:To prepare anti-VSIG4 monoclonal antibodies and characterize their biological functions.Methods:BALB/c mice were immunized with transfected cell line (L929/VSIG4L) as immunogen.The spleen B cells of the mice were fused with SP2/0 and hybridoma cells were screened with transfected cell line (L929/VSIG4) by FCM.After acquisition of the hybridomas secreting anti-VSIG4 mAb,their biological activities were investigated by indirect immunofluorescence,Western blot,competitive inhibition test,and MTT assa... |
| |
| Keywords: | VSIG4 VSIG4 Hybridoma Monoclonal antibody Macrophage |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
