| 益肾活血饮逆转骨髓瘤凋亡抵抗的研究 |
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| 引用本文： | 胡永珍,李达,韩凌.益肾活血饮逆转骨髓瘤凋亡抵抗的研究[J].深圳中西医结合杂志,2012,22(1):1-3,20. |
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| 作者姓名： | 胡永珍 李达 韩凌 |
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| 作者单位： | 广东省中医院,广东广州,510120 |
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| 基金项目： | 广东省中医药管理局资助项目(2010141) |
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| 摘 要： | 目的:探讨中药益肾活血饮对骨髓瘤凋亡的影响及其机理。方法:以阿霉素共培养的骨髓瘤细胞上清作为条件培养液诱发凋亡抵抗(化疗后组),再加入益肾活血饮含药血清(中药+化疗后组),观察其对骨髓瘤细胞HIF-1α表达(免疫印迹法)及继发凋亡抵抗(流式细胞术)的影响。结果:化疗后组骨髓瘤细胞HIF-1α表达上升,凋亡率下降。中药+化疗后组HIF-1α表达低于化疗后组,但较中药组及空白组高;其凋亡率则高于化疗后组,但较中药组及空白组低。结论:益肾活血饮可能通过下调HIF-1,部分逆转骨髓瘤细胞的继发凋亡抵抗。
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| 关 键 词： | 益肾活血饮 骨髓瘤 阿霉素 凋亡抵抗 低氧诱导因子 |
Effect of Yi-Shen-Huo-Xue Decoction on Apoptosis Resistance of Multiple Myeloma |
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| HU Yong-zhen
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LI Da
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HAN Ling.Effect of Yi-Shen-Huo-Xue Decoction on Apoptosis Resistance of Multiple Myeloma[J].Shenzhen Journal of Integrated Traditional Chinese and Western Medicine,2012,22(1):1-3,20. |
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| Authors: | HU Yong-zhen LI Da HAN Ling |
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| Institution: | (Guangdong Provincial Hospital of TCM,Guangdong Guangzhou 510120) |
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| Abstract: | Objective To explore the effect of traditional Chinese medicine(TCM) Yi-Shen-Huo-Xue Decoction on multiple myeloma apoptosis resistance.Methods Multiple myeloma cell U266 was incubated with chemotherapy drug adriamycin to induce apoptosis resistance(Postchemotherapy group),with(TCM+Postch-emotherapy group) or without adding of Chinese medicine decoction serum.HIF-1α protein level and apoptosis ratio were analyzed by western blot and flow cytometry respectively.Results Adriamycin enhanced the expression of HIF-1α,and induced apoptosis resistance in multiple myeloma cells,traditional Chinese medicine decoction partly blocked HIF-1α expression and reversed the apoptosis resistance.Conclusion Traditional Chinese medicine Yi-Shen-Huo-Xue Decoction can partly reverse multiple myeloma apoptosis mediated by HIF-1. |
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| Keywords: | Yi-Shen-Huo-Xue Decotion Multiple myeloma Adriamycin Apoptosis resistance Hypoxia-inducible factor-1(HIF-1) |
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