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Induction of apoptosis of human gastric carcinoma SGC-7901 cell line by 5, 7-dihydroxy-8-nitrochrysin in vitro |
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| Authors: | Ai Xiao-Hong Zheng Xing Tang Xiao-Qing Sun Li Zhang Yang-Qin Qin Yong Liu Hua-Qing Xia Hong Cao Jian-Guo |
| Institution: | 1. Department of Oncology, First Affiliated Hospital,Nahua University, Hengyang 421001, Hunan Province, China;Institute of Oncology, Nahua University, Hengyang 421001, Hunan Province,China 2. Institute of Pharmacology, Nahua University, Hengyang 421001, Hunan Province, China 3. Institute of Oncology, Nahua University, Hengyang 421001, Hunan Province,China;Institute of Pharmacology, Nahua University, Hengyang 421001, Hunan Province, China;School of Pharmaceutical Science, Central South University, Changsha 410078, Hunan Province, China 4. Institute of Oncology, Nahua University, Hengyang 421001, Hunan Province,China;Institute of Pharmacology, Nahua University, Hengyang 421001, Hunan Province, China |
| Abstract: | AIM: To investigate the effect of 5, 7-dihydroxy-8-nitrochrysin (NOChR) on apoptosis of human gastric carcinoma SGC-7901 cell line. METHODS: SGC-7901 cells were cultured in vitro and the inhibitory effect of NOChR on proliferation of SGC-7901 cells was measured by using an MTT assay. NOChR-induced apoptosis rate of SGC-7901 cells was detected using flow cytometry (FCM) with PI staining. DNA ladder bands were observed by DNA agarose gel electrophoresis. The influence of NOChR on the proxisome proliferator-activated receptor-gamma (PPARgamma), Bcl-2 and Bax protein expression of SGC-7901 cells was analyzed by Western blot. RESULTS: MTT assay showed that NOChR markedly inhibited proliferation of SGC-7901 cells in a dose-dependent manner, and when IC(50) was 4.14 micromol/L, the potency of NOChR was 10 times than that of lead compound, chrysin (ChR, IC(50) was 40.56 micromol/L), and was similar to 5-fluorouracil (5-FU, IC(50) was 4.51 micromol/L). FCM with propidium iodide (PI) staining demonstrated that the apoptosis rates of SGC-7901 cells treated with 1.25, 5.00 and 20.00 micromol/L NOChR for 48 h were 9.8% +/- 0.2%, 36.8% +/- 1.9% and 45.5% +/- 3.5%, respectively, and were significantly higher when treated with 5.00 and 20.00 micromol/L NOChR than that with 20.00 micromol/L ChR (12.9% +/- 1.5%). DNA agarose gel electrophoresis showed that treatment of SGC-7901 cells with 20.00 micromol/L NOChR for 48 h resulted in typical DNA ladder bands of DNA of SGC-7901 cells, which could be eliminated by treating with 10.00 micromol/L GW9662, a blocker of PPARgamma. Western blot analysis revealed that after 24 h of treatment with 20.00 micromol/L NOChR, PPARgamma and Bax protein expression of SGC-7901 cells increased but Bcl-2 expression decreased; however, pre-incubation with 10.00 micromol/L GW9662 could efficiently antagonize and weaken the regulatory effect of 20.00 micromol/L NOChR on Bax and Bcl-2 protein expression of SGC-7901 cells. |
| Keywords: | Gastric neoplasm Chrysin Chrysin derivatives Apoptosis Bcl-2 Bax |
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