Ki-67蛋白在人骨肉瘤中表达的研究

目的:探讨ki-67蛋白在人骨肉瘤中的表达及其与骨肉瘤发生、发展和病理分型的关系。方法:采用免疫组化S-P蛋白的表达情况。结果:ki-67蛋白在骨肉瘤中阳性表达率为66.67％,在骨软骨瘤中阳性表达率为10％,比较骨肉瘤和骨软骨瘤染色阳性率和染色强度,差异均有显著性(P<0.05)。ki-67蛋白在骨肉瘤高分化组阳性表达率为28.57％,在骨肉瘤低分化组的阳性表达率为78.26％,两组染色阳性率及染色强度的差异均有显著性(P<0.05)。而ki-67蛋白在骨肉瘤各病理亚型的染色结果,差异无显著性(P>0.05)。结论:ki-67蛋白表达与骨肉瘤发生、发展密切相关,其阳性表达率和表达程度是判断骨肉瘤恶性程度的一个指标。

Expression of ki-67 protein in human osteosarcoma

objective: To evaluate the expression of ki-67 protein and its relation to carcinogenesis, development and pathological types of osteosarcoma . Methods: S-P immunohistochemical method was used to detect expression of ki-67 protein in 30 cases of osteosarcoma and 10 cases of osteochondroma. Results: The positive rate oj ki-67 protein was 66.67% in osteosarcoma and 10% in osteochondroma , comparing thf staining rate and intensity between them , respectively, there were all significant differences (P<0.05). The positive immunostaining rate was 28.57% in higher differentiation group and 78.26% in lower differentiation group, comparing the staining rate and intensity oj higher and lower differentiation , respectively, there were all significant differences ( P<0.05) . Comparing the staining results of the pathological types, there all were not significant differences (P>0.05). Conclusion: The expression of ki-67 protein in deeply involved in the carcinogenesis and development of osteosarcoma . It can be a marker of malignant grade oj osteosarcoma .