应用活体冷冻技术动态观察各种血流动力学改变时血清白蛋白在小鼠近端肾小管的分布变化

目的 应用活体冷冻技术结合免疫组织化学的方法形象化表现各种血流动力学条件下小鼠血清白蛋白经肾小球毛细血管袢滤过后,在近曲小管再吸收的病理生理过程.方法 分别在正常血流、急性高血压和心脏骤停等血流动力学条件下,对模型小鼠的左肾进行活体冷冻及冷冻置换.连续的石蜡切片后,分别进行苏木精一伊红染色和抗小鼠白蛋白抗体免疫组织化学染色.结果 正常血压和心脏骤停情况下,血清白蛋白在血管内清晰定位,少量出现在近端小管顶端的细胞质部分,但在急性高血压情况下,血清白蛋白明显地免疫定位于所有近端小管.结论 活体冷冻技术结合冷冻置换方法可以原位捕获瞬间被活体功能状态的近端肾小管上皮细胞再吸收的血清白蛋白,可视化活体组织细胞的功能形态学特征.

Observation of serum albumin distribution on renal proximal tubules under various hemodynamic conditions in mice by "in vivo cryotechnique"

Objective To visualize the topographical changes of serum albumin passing through mouse glomerular capillary loops and its reabsorption in renal proximal tubules by immunohistochemistry in combination with "in vivo cryotechnique".Methods The "in vivo cryotechnique" was used to treat the left kidneys of mice under normotensive,experimentally acute hypertensive and heart-arrest conditions. The cryofixed tissues were routinely processed for freeze-substitution. Serial deparaffinized sections were stained with ...