| 脂肪组织切片制备方法的改进 |
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| 引用本文： | 徐玉环.脂肪组织切片制备方法的改进[J].中国比较医学杂志,2017,27(1):79-84. |
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| 作者姓名： | 徐玉环 |
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| 作者单位： | 中国医学科学院医学实验动物研究所, 卫生部人类疾病比较医学重点实验室, 国家中医药管理局人类 疾病动物模型三级实验室, 新发再发传染病动物模型研究北京市重点实验室, 北京 100021;中国医学科学院医学实验动物研究所, 卫生部人类疾病比较医学重点实验室, 国家中医药管理局人类 疾病动物模型三级实验室, 新发再发传染病动物模型研究北京市重点实验室, 北京 100021;中国医学科学院医学实验动物研究所, 卫生部人类疾病比较医学重点实验室, 国家中医药管理局人类 疾病动物模型三级实验室, 新发再发传染病动物模型研究北京市重点实验室, 北京 100021;中国医学科学院医学实验动物研究所, 卫生部人类疾病比较医学重点实验室, 国家中医药管理局人类 疾病动物模型三级实验室, 新发再发传染病动物模型研究北京市重点实验室, 北京 100021;中国医学科学院医学实验动物研究所, 卫生部人类疾病比较医学重点实验室, 国家中医药管理局人类 疾病动物模型三级实验室, 新发再发传染病动物模型研究北京市重点实验室, 北京 100021;中国医学科学院医学实验动物研究所, 卫生部人类疾病比较医学重点实验室, 国家中医药管理局人类 疾病动物模型三级实验室, 新发再发传染病动物模型研究北京市重点实验室, 北京 100021;中国医学科学院医学实验动物研究所, 卫生部人类疾病比较医学重点实验室, 国家中医药管理局人类 疾病动物模型三级实验室, 新发再发传染病动物模型研究北京市重点实验室, 北京 100021;中国医学科学院医学实验动物研究所, 卫生部人类疾病比较医学重点实验室, 国家中医药管理局人类 疾病动物模型三级实验室, 新发再发传染病动物模型研究北京市重点实验室, 北京 100021;中国医学科学院医学实验动物研究所, 卫生部人类疾病比较医学重点实验室, 国家中医药管理局人类 疾病动物模型三级实验室, 新发再发传染病动物模型研究北京市重点实验室, 北京 100021;中国医学科学院医学实验动物研究所, 卫生部人类疾病比较医学重点实验室, 国家中医药管理局人类 疾病动物模型三级实验室, 新发再发传染病动物模型研究北京市重点实验室, 北京 100021;中国医学科学院医学实验动物研究所, 卫生部人类疾病比较医学重点实验室, 国家中医药管理局人类 疾病动物模型三级实验室, 新发再发传染病动物模型研究北京市重点实验室, 北京 100021 |
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| 基金项目： | 国家自然科学基金面上项目（81371546）。 |
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| 摘 要： | 目的探讨脂肪组织石蜡切片及冰冻切片方法的改进。方法取脂肪组织迅速固定于10%中性福尔马林中,设置不同的脱水程序制备石蜡切片,HE染色。免疫组化法观察PPARγ和ITLN1的表达。取脂肪组织迅速固定于甲醛钙或10%中性福尔马林固定液中24 h以上,OCT包埋,置于-80℃冰箱至少30 min,设置冰冻切片机箱体温度-20℃,样品头温度-30℃,切片后HE染色。结果用改进方法制备的脂肪组织石蜡切片,切面平整,无皱褶,无裂隙,脂肪细胞结构完整,染色清晰,且对免疫组化结果无影响;冰冻切片的改进提高了切片质量,结构完整,形态好,染色清晰。结论改进的脂肪组织石蜡切片和冰冻切片均可成功应用于不同实验动物,为脂肪组织的研究提供了有力的支撑条件。
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| 关 键 词： | 脂肪组织 石蜡切片 冰冻切片 |
| 收稿时间： | 2016/6/17 0:00:00 |
| 修稿时间： | 2016/12/13 0:00:00 |
A modification of adipose tissue preparation |
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| xuyuhuan.A modification of adipose tissue preparation[J].Chinese Journal of Comparative Medicine,2017,27(1):79-84. |
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| Authors: | xuyuhuan |
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| Institution: | Institute of Laboratory Animal Science, Chinese Academy of medical Sciences. Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, Key Laboratory of Human Diseases Animal Model, State Administration of Traditional Chinese Medicine, Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious Diseases, Beijing 100021, China;Institute of Laboratory Animal Science, Chinese Academy of medical Sciences. Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, Key Laboratory of Human Diseases Animal Model, State Administration of Traditional Chinese Medicine, Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious Diseases, Beijing 100021, China;Institute of Laboratory Animal Science, Chinese Academy of medical Sciences. Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, Key Laboratory of Human Diseases Animal Model, State Administration of Traditional Chinese Medicine, Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious Diseases, Beijing 100021, China;Institute of Laboratory Animal Science, Chinese Academy of medical Sciences. Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, Key Laboratory of Human Diseases Animal Model, State Administration of Traditional Chinese Medicine, Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious Diseases, Beijing 100021, China;Institute of Laboratory Animal Science, Chinese Academy of medical Sciences. Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, Key Laboratory of Human Diseases Animal Model, State Administration of Traditional Chinese Medicine, Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious Diseases, Beijing 100021, China;Institute of Laboratory Animal Science, Chinese Academy of medical Sciences. Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, Key Laboratory of Human Diseases Animal Model, State Administration of Traditional Chinese Medicine, Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious Diseases, Beijing 100021, China;Institute of Laboratory Animal Science, Chinese Academy of medical Sciences. Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, Key Laboratory of Human Diseases Animal Model, State Administration of Traditional Chinese Medicine, Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious Diseases, Beijing 100021, China;Institute of Laboratory Animal Science, Chinese Academy of medical Sciences. Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, Key Laboratory of Human Diseases Animal Model, State Administration of Traditional Chinese Medicine, Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious Diseases, Beijing 100021, China;Institute of Laboratory Animal Science, Chinese Academy of medical Sciences. Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, Key Laboratory of Human Diseases Animal Model, State Administration of Traditional Chinese Medicine, Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious Diseases, Beijing 100021, China;Institute of Laboratory Animal Science, Chinese Academy of medical Sciences. Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, Key Laboratory of Human Diseases Animal Model, State Administration of Traditional Chinese Medicine, Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious Diseases, Beijing 100021, China;Institute of Laboratory Animal Science, Chinese Academy of medical Sciences. Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, Key Laboratory of Human Diseases Animal Model, State Administration of Traditional Chinese Medicine, Beijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious Diseases, Beijing 100021, China |
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| Abstract: | |
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| Keywords: | Adipose tissues Paraffin sections Frozen sections |
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