脑缺血再灌注后脑组织c—fos基因表达与东菱克栓酶的影响

本实验采用线栓法制成大鼠大脑中动脉缺血再灌注模型,用地高辛精标记的c—fos探针进行原位杂交。结果示缺血再灌注组栓塞侧皮层及海马c—fos基因表达显著增多,图像分析灰阶值为118.6±5.1,对侧为159.6±3.1(P<0.001),东菱克栓酶组栓塞侧皮层及海马c—fos基因表达亦增多,灰阶为131.0±4.5,对侧为164.6±4.0(P<0.001)。克栓酶组与缺血再灌注组比较,栓塞侧东菱克栓酶组c—fos基因表达显示低于缺血再灌组(P<0.05),而两组栓塞对侧比较无显著差异。本实验表明,脑缺血再灌注后脑组织的c—fos基因表达显著增多,东菱克栓酶能抑制缺血后c—fos基因的表达,这可能是其治疗缺血性脑血管病的机理之一。

Effect of batroxobin on c-fos gene expression in rat brains after cerebral ischemia and reperfusion

The local cerebral ischemia and reperfusion model of rats was made with thread embolism of middle cerebral atery. After 60 min reperfusion following 90 min ischemia, c - fos mR-NA expression in rat brain was investigatied with in situ hybridization and batroxobin - treated group. The results showed that c -fos gene expression in cerebral cortex and hippocampus of ischemic sides was significantly increased as compared nonischemic sides (P<0. 01). Although c - fos gene expression in batroxobin treated - rat was also increased, yet it was significantly lower than those of ischemia following reperfusion group(p<0. 05). The results indicated that c -fos gene-expression in rat brain can be induced by reperfusion following cerebral ischemia and botroxobin may partly play a role in inhibition of c -fos gene expression during ischemia and reperfusion.