多发性硬化症髓鞘素组分特异性抗体分泌细胞

本文用酶联免疫斑点法（Ｅｌｉｓｐｏｔ）检测了２３例临床确诊多发性硬化症（ＭＳ）和１２例无菌性脑膜炎（ＡＭ）患者外周血（ＰＢ）和脑脊液（ＣＳＦ）中髓鞘素碱性蛋白（ＭＢＰ）、髓鞘素结合糖蛋白（ＭＡＧ）和含脂质蛋白（ＰＬＰ）特异性ＩｇＧ抗体分泌细胞。两组患者ＣＳＦ中该３种抗体分泌细胞均呈明显增多趋势，ＭＳ组尤著，但两组ＰＢ中该类细胞数均很少。指示对髓鞘素组分的Ｂ细胞免疫应答主要局限于与中枢神经系统（ＣＮ

Specific anti-myelin component antibody secreting cells in multiple sclerosis

The specific B cell response against myelin basic protein (MBP),myelin associated protein (MAG) and myelin prote-olipid protein (PLP) was evaluated in peripheral blood and cerebrospinal fluid (CSF) 23 patients with multiple sclerosis (MS) and 12 control patients with aseptic meningitis (AM) by enumeration of cells secreting anti-MBP,anti-MAG and an-ti-PLP antibodies of IgG isotype. We used a nitrocellulose mmunospot assay which enables enumeration of numbers of cells secretion these specific antibodies. The numbers of 3 antibody secreting cells in SF from patients with MS and AM were elevated.especially in MS patients,but a few numbers of cells were found in peripheral blood in both groups. The results revealed that the B cell response against myelin components is preferentially sequestered to CSF compartment which is close to central nervous system and probably the important source of intrathecal IgG synthesis.