Effect of peripheral 2-DG on opioid and neuropeptide Y gene expression |
| |
Authors: | Silvia Q Giraudo Eun-Mee Kim Martha K Grace Charles J Billington Allen S Levine |
| |
Affiliation: | Minnesota Obesity Center, V.A. Medical Center, Departments of Medicine and Food Science and Nutrition, University of Minnesota, Minneapolis and St. Paul, MN 55417, USA |
| |
Abstract: | It is well known that 2-Deoxy-d-glucose (2-DG) blocks intracellular utilization of glucose and increases food intake. The aim of the present study was to determine whether administration of 2-DG alters gene expression of the orexigenic peptides, neuropeptide Y (NPY) and endogenous opioids, in the arcuate nucleus of the hypothalamus (ARC). Male Sprague–Dawley rats were injected peripherally (i.p.) with 2-DG (200 or 400 mg/kg body weight) and were sacrificed at 2 or 6 h post injection. Half of the animals were given ad libitum access to food whereas the other half of the animals were food-deprived. 2-DG increased food intake fourfold compared to saline injected animals, but did not affect NPY mRNA levels after 2 h. Messenger RNA levels of ProDynorphin (proDYN), but not pro-opiomelanocortin (POMC) nor proEnkephalin (proENK) were significantly decreased 2 h after 2-DG injection. Administration of 400 mg/kg of 2-DG increased mRNA levels of NPY in the arcuate nucleus after six h, but only in those animals not receiving food. |
| |
Keywords: | Neuropeptide Y (NPY) proDynorphin (proDYN) proEnkephalin (proENK) pro-opiomelanocortin (POMC) 2-deoxy- font-variant: small-caps" >d-glucose (2-DG) Periphery |
本文献已被 ScienceDirect 等数据库收录! |
|