声门上型喉鳞癌微淋巴管密度检测及其临床意义

背景与目的：肿瘤微淋巴管可能对肿瘤的转移起重要作用。本研究旨在通过检测声门上型喉鳞癌微淋巴管密度（1ymphatic microvessel density，LVD），了解声门上型喉鳞癌微淋巴管生成与其临床病理因素的关系，评估LVD对判断声门上型喉癌预后的价值。方法：回顾性分析确诊并行原发灶切除的51例声门上型喉鳞癌患者临床病理资料，取原发灶石蜡切片行podoplanin单克隆抗体免疫组化染色，并计数podoplanin染色阳性的LVD，统计分析其与喉鳞癌临床病理因素及预后的关系。结果：51例声门上型喉癌标本LVD均值为3．25±2．37，中位数为3，有颈淋巴结转移组患者LVD较无转移组高，差异具有统计学意义（P=0．009）。总体5年生存率为54．9％，肿瘤低分化、局部晚期、淋巴结转移、临床晚期及LVD高均可使5年生存率下降。Cox回归分析提示肿瘤病理分化、T分期及LVD是声门上型喉鳞癌的独立预后因素。结论：声门上型喉癌LVD与淋巴结转移及预后相关，是声门上型喉癌的独立预后因素。

Detection and clinical significance of lymphatic microvessel density in supraglottic laryngeal carcinoma

Background and important role in tumor microvessel density （LVD） objective： metastasis Lymphatic microvessels may play an This study detected n supraglottic laryngeal carcinoma lymphatic to find its correlation to clinicopathologic characteristics, and to assess its prognostic significance in supraglottic laryngeal carcinoma. Methods. Clinical data of 51 supraglottic laryngeal carcinoma patients who underwent primary lesion resection were reviewed. Immunohistochemicat staining was performed with podoplanin monoclonal antibody to recognize lymphatic microvessels. The correlations of LVD to clinicopathologic characteristics and prognosis were analyzed. Results. The mean LVD was 3.25＋2.37, with a median of 3, in the 51 specimens of supraglottic laryngeal carcinoma. LVD was significantly hJgher Jn the patients with cervical lymph node metastasis than in those without （P=O.O09）. The overall 5-year survival rate was 54.9%. The 5-year survival rate was significantly lower in the patients with poorly differentiated tumor, stages T3-4 disease, lymph node metastasis, stages Ⅲ-Ⅳ disease and high LVD than in their counterparts. Cox regression analysis revealed that LVD, T classification, and differentiation were independent factors for survival. Conclusion. LVD is related to lymph nodes status, and is an independent factor for prognosis of supraglottic laryngeal carcinoma.