银屑病患者皮损及鳞屑中人乳头瘤病毒的检测

目的 检测银屑病患者皮损中人乳头瘤病毒(HPV) DNA，探讨其与银屑病的关系。方法 选取3组通用引物，用聚合酶链反应(PCR)检测银屑病活检标本(62份)、皮损处刮屑标本(79份)、非皮损处刮屑标本(62份)、正常人对照的活检标本(48份)和刮屑标本(48份)中的HPV DNA。结果 疣状表皮发育不良相关性人乳头瘤病毒(EV HPV)的DNA检出率在银屑病皮损活检标本中为77.4%，而在正常人活检标本中为27.1%，差异有显著性(P < 0.005)。在银屑病患者的皮损和非皮损处的刮屑中，EV HPV DNA检出率分别为51.9%和48.4%。而正常人刮屑标本中为18.8%，与前两者差异均有显著性(P < 0.005)。在所有标本中均未检测到嗜黏膜性HPV DNA。 结论 银屑病患者中EV HPV DNA的检出率远高于正常人。

Detection of human papillomavirus in psoriatic lesions and scrapings

Objective: To detect DNA of human papillomavirus (HPV) in psoriatic skin and find out the possible relationship between HPV and psoriasis. Methods: Three groups of consensus primers were used in the polymerase chain reaction (PCR) to detect HPV DNA in 62 biopsy and 79 scraping specimens from psoriatic lesions and 62 scrapings from non-lesion skin of psoriatic patients. Take both biopsy and scraping specimens of 48 non-psoriatic plastic surgery patients as controls. Results: Epidermodysplasia verruciformis-associated human papillomavirus (EV HPV) DNA was found in 77.4% psoriatic biopsies, versus only 27.1% in the controls (P < 0.005). The detection rates in the scrapings from psoriatic lesions and non-lesions were 51.9% and 48.4%; versus only 18.8% in the controls (P < 0.005). No mucosal type was detected in all the samples. Conclusion: EV HPV DNA was detected more frequently in psoriatic skin than in healthy control.