动物源性大肠埃希菌超广谱β-内酰胺酶的检测及耐药性研究

目的了解动物源性大肠埃希菌超广谱β-内酰胺酶（ESBLs）的检出率和耐药特点,为其耐药性的安全评价提供依据。方法采用NCCLS推荐的表型检测法和确认试验测定大肠埃希菌ESBLs,纸片扩散法测定大肠埃希菌的耐药性。结果 222株大肠埃希菌对环丙沙星（83.78%）、诺氟沙星（92.79%）、利福平（81.08%）的耐药率很高;对阿米卡星、亚胺培南高度敏感。ESBLs检出率为12.16%（27/222）,产ESBLs菌株除对亚胺培南外其余13种抗生素的耐药率均高于非产ESBLs菌株。结论动物源性大肠埃希菌对很多抗生素具有一定的耐药性,应加强对食品中动物源性大肠埃希菌耐药性的监测。

ESBLs detection and drug resistance study on Escherichia coli of animal origin

Objective To investigate the detection rate and the drug resistant characteristic of ESBLs on Escherichia coli of animal origin in order to provide a basis for the safety evaluation of drug resistance. Methods ESBLs of Escherichia coli was detected by phenotype detection method and confirmatory test recommended by NCCLS.Drug resistance of Escherichia coli was detected by disk diffusion method. Results The drug resistant rates of 222 Escherichia coli strains on ciprofloxacin（83.78%）,noroxin（92.79%）and rifampicin（81.08%）were quite high,while the strains were high sensitive to amikacin and imine culture south.The detection rate of ESBLs was 12.16%（27/222）.In addition to imipenem,the drug resistant rate of ESBLs-producing strains on 13 kinds of antimicrobials was much higher than that of nonESBLs-producing strains respectively. Conclusion Escherichia coli of animal origin have certain resistance to a great many antibiotics.It should strengthen the monitoring of drug resistance on Escherichia coli of animal origin in the food.