“寒热并见”大鼠模型血清三碘甲腺原氨酸、甲状腺素含量变化研究

目的观察“寒热并见”模型大鼠血清三碘甲腺原氨酸（T3）、甲状腺素（T4）的含量变化，探讨其作为“寒热并见”动物模型寒证指标的意义。方法采用丙硫氧嘧啶和寒凉中药灌胃配合冷冻的多因素复合造模法制作虚寒证模型，在此基础上，皮下注射干酵母混悬液致大鼠发热，造成约40h的“寒热并见”动物模型。检测甲状腺激素耶，T4含量。结果与正常组比较，“寒热并见”模型大鼠血清13，T4含量显著下降。结论“寒热并见”模型大鼠血清T3，T4含量下降，表明机体表现出虚寒状态，虽然“寒热并见”模型大鼠表现为发热，但实际为表热里寒。

Study on the changes of the contents of serum T3 and T4 in rat model with cold-heat complication syndrome

Objective To observe the changes of the contents of serum T3 and T4 in rat model with cold-heat complication syndrome, discuss the meaning of T3 and T4 as a index of the cold-heat complication syndrome. Methods The model of deficiency-cold syndrome was made by multi-factor composite method including gavage of propyhhiouracil and cold drugs and frozening rats, making a deficiency cold syndrome model, on the base of this, made the rats fever by injecting dried yeast suspension at subcutaneous, and created the animal model with cold-heat complication syndrome for about 40 hours. Detected serum 33 and T4. Results Compared with normal group, the contents of serum T3, T4 of the rats in cold-heat complication syndrome group were significantly reduced. Conclusion The decrease of contents of serum T3 and T4 shows that the body put up asthenia cold state, although the rat model with cold-heat complication syndrome show fever, but it is superficial heat with interior cold.