| 应用RNAi技术沉默survivin基因对肾癌786-O细胞的影响 |
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| 引用本文： | 李戈,王明.应用RNAi技术沉默survivin基因对肾癌786-O细胞的影响[J].第三军医大学学报,2009,31(24):2444-2447. |
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| 作者姓名： | 李戈 王明 |
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| 作者单位： | 重庆医科大学附属第一医院泌尿外科,重庆,400016 |
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| 摘 要： | 目的 运用RNA干扰(RNA interference,RNAi)技术特异性阻断肾癌786-O细胞系中survivin基凶的表达,并研究survivin沉默后对786-O细胞的影响.方法 用真核转录载体pcDNA~(TM)6.2-GW/EmGFPmiR构建针对survivin基因的干扰载体,用脂质体法转染肾癌786-O细胞,通过RT-PCR、免疫组化分别检测survivin基因的mRNA和蛋白水平的表达,MTT法检测细胞生长的影响.结果 干扰载体有效地阻断了786-O细胞中survivin基因在mRNA与蛋白水平上的表达,细胞活性受到抑制.结论 RNAi技术沉默survivin基因可以下调肾癌786-O细胞survivin基因的表达,抑制细胞的活性.
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| 关 键 词： | RNA干扰 肾癌 基因治疗 |
Silencing survivin gene with RNAi inhibits proliferation of human renal cancer cell line 786-O |
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| LI Ge,WANG Ming.Silencing survivin gene with RNAi inhibits proliferation of human renal cancer cell line 786-O[J].Acta Academiae Medicinae Militaris Tertiae,2009,31(24):2444-2447. |
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| Authors: | LI Ge WANG Ming |
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| Institution: | LI Ge,WANG Ming (Department of Urinary Surgery,First Affiliated Hospital,Chongqing Medical University,Chongqing 400016,China) |
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| Abstract: | Objective To explore the inhibitory effects of RNAi targeting survivin to suppress the expression of survivin and to induce the apoptosis of human renal cancer cell line 786-O.Methods The target gene fragments were cloned into pcDNATM 6.2-GW/EmGFPmiR vectors,and then the recombinant eukaryotic expression vectors were transfected into 786-O cells with liposomes.The expression changes in survivin mRNA and protein were detected by RT-PCR and immunohistochemical assay,and the proliferation inhibitory rate (IR) ... |
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| Keywords: | survivin RNA interference surviving renal cancer gene therapy |
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