Procedure for concentration and conditioning of hybridoma cell culture medium for monoclonal antibody purification |
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| Authors: | K. Veeraragavan A. Bernier M. Oliver E. Braendli |
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| Affiliation: | (1) BioSeparation Group, Biochemical Engineering Section, Biotechnology Research Institute, National Research Council of Canada, H4P 2R2 Montreal, Canada |
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| Abstract: | Summary Concentration and conditioning the hybridoma cell culture medium is an important step in the monoclonal antibody purification procedure. This report describes a frequent first-step concentration of the hybridoma cell culture medium and the conditioning of the concentrated medium for the affinity purification of the monoclonal antibodies. |
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| Keywords: | hybridoma culture medium concentration conditioning monoclonal antibody purification |
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