肾虚型老年痴呆动物模型的建立

目的探讨通过皮下注射氢化可的松复合侧脑室注射β-淀粉样蛋白(Aβ)建立肾虚型老年痴呆动物模型的方法,为抗痴呆中药新药的研究提供实验模型。方法分别给予动物皮下注射氢化可的松、侧脑室注射β-淀粉样蛋白25-35(Aβ25-35)、皮下注射氢化可的松复合侧脑室注射Aβ25-35,通过体质量变化、跑步力竭时间、免疫学指标、血清皮质酮水平变化评价是否造成动物肾虚,通过morris水迷宫测试、脑组织病理学变化评价是否造成痴呆。结果与对照组相比,氢化可的松组动物出现体质量增长缓慢,自主活动减少,水迷宫上台潜伏期稍有延长,跑步力竭时间缩短,胸腺和脾指数降低,脾细胞刺激指数降低,血清皮质酮值降低;脑室注射Aβ的动物水迷宫上台潜伏期延长,但不出现前述的一系列肾虚表现;皮下注射氢化可的松复合侧脑室注射Aβ6μg的动物既出现肾虚的表现又出现学习记忆障碍;皮下注射氢化可的松复合侧脑室注射Aβ3μg也造成动物的肾虚表现,但并未造成严重的学习记忆障碍。结论皮下注射氢化可的松造成动物肾虚表现,侧脑室注射Aβ25-356μg可造成动物学习记忆障碍,皮下注射氢化可的松复合侧脑室注射Aβ25-356μg的动物既有肾虚证的表现也有学习记忆障碍,认为造成了肾虚型老年痴呆模型。

Establish of Kidney deficient Alzheimer's mouse model

Objectives It is to explore how to establish the kidney-deficient Alzheimer’s mouse model by hypodermic inject Hydrocortisone and lateral cerebral ventricle inject Amyloid β-Protein(Aβ),so to provide experimental models for the study on new anti-dementia Chinese drugs.Methods KM mice were randomly divided and treated respectively as follows: hypodermic injected with Hydrocortisone,lateral cerebral ventricle inject with Aβ25-35,hypodermic injected with Hydrocortisone and lateral cerebral ventricle inject with Aβ25-35.Whether Kidney-deficient was induced were diagnozed by detection of body weight,exhaustive running time immunological indexes,serum corticosterone.Amnesia degrees were evaluated by morris water maze test and pathological change of cerebral tissue.Results Compared with the control group,the Hydrocortisone group was kidney-deficient as weight grow slowly,autonomic activity decreased,latency of seeking the platform in morris water maze prolonged a little,exhaustive running time shortened,thymus index,spleen index and spleen cell stimulation index decreased,serum corticosterone reduced.The animals given Aβ by lateral cerebral ventricle injection had prolonged latency of seeking the platform in morris water maze without Kidney deficient syndromes mentioned above.The Hydrocortisone+ Aβ 6μg group had both the behaviors of kidney-deficient and amnesia,but the ones given Hydrocortisone+ Aβ 3μg only had kidney-deficient,their amnesia syndromes were not obvious.Conclusion Injection with Hydrocortisone can induce Kidney-deficient syndrome in the animals.Lateral cerebral ventricle injection with Aβ25-35 6μg can induce amnesia.Hypodermic injection with Hydrocortisone and lateral cerebral ventricle injection with Aβ25-35 6μg can induce Kidney-deficient syndromes and learning-memory disorders,so this method can establish kidney-deficient Alzheimer’s mouse model.