| 宫内发育迟缓对大鼠脑内NGF及TrkA表达影响的研究 |
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| 引用本文： | 曲香,贺宝平,王华.宫内发育迟缓对大鼠脑内NGF及TrkA表达影响的研究[J].中国当代儿科杂志,2011,13(5):415-418. |
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| 作者姓名： | 曲香 贺宝平 王华 |
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| 作者单位： | 曲香，贺宝平，王华 |
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| 摘 要： | 目的：观察神经生长因子（NGF）及酪氨酸激酶受体A（TrkA）在宫内发育迟缓(IUGR)大鼠脑不同发育阶段的变化，探讨IUGR大鼠脑发育迟缓的机制。方法：32只孕鼠随机分成 IUGR 组和正常对照组。采用孕期全程低蛋白饮食的方法建立IUGR大鼠模型。各组仔鼠于生后 0 d、7 d、14 d、21 d断头取脑，免疫组织化学及 Western blot 方法检测脑中 NGF 和 TrkA 蛋白的表达情况。结果：免疫组化及Western blot结果均显示低蛋白饮食组0 d、7 d、14 d、21 d仔鼠脑内 NGF 及 TrkA 蛋白的表达均较正常组显著降低，差异有统计学意义（P<0.01）。结论：脑内NGF 及TrkA蛋白表达的降低可能是IUGR大鼠脑发育迟缓发生的机制之一。
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| 关 键 词： | 宫内发育迟缓 神经生长因子 酪氨酸激酶受体 大鼠 |
Expression of NGF and TrkA in the brain of rats with intrauterine growth retardation |
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| QU Xiang,HE Bao-Ping,WANG Hua.Expression of NGF and TrkA in the brain of rats with intrauterine growth retardation[J].Chinese Journal of Contemporary Pediatrics,2011,13(5):415-418. |
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| Authors: | QU Xiang HE Bao-Ping WANG Hua |
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| Institution: | QU Xiang, HE Bao-Ping, WANG Hua |
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| Abstract: | Objective To study the mechanism of brain development delay in rats with intrauterine growth retardation(IUGR) by examining the expression of nerve growth factor(NGF) and tyrosine kinase receptor A(Trk A) in the brain.Methods Thirty-two pregnant rats were randomly fed with a normal diet(control group) or lower protein diet(IUGR group) during pregnancy(n=16 each).The pup rats were sacrificed at 0,7,14 or 21 days after birth.The protein levels of NGF and TrkA in the brain were determined by Western blot and i... |
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| Keywords: | Intrauterine growth retardation Nerve growth factor Tyrosine kinase receptor A Rats |
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