柴胡总皂苷醇洗脱精制品对大鼠急性毒性实验研究

目的探讨柴胡总皂苷醇洗脱精制品对大鼠急性毒性的影响。方法采用经典的急性毒性试验方法,进行柴胡总皂苷醇洗脱精制品对大鼠的急性毒性实验研究。结果按含柴胡总皂苷含量计算,柴胡总皂苷醇洗脱精制品对小鼠的半数致死量（LD50）为591.95mg·kg-1·d-1,95%可信限为490.96～713.7mg·kg-1·d-1。死亡大鼠急性毒性主要表现为安静怠动,即而出现步态不稳、腹卧昏睡、心率加快、呼吸急促、连续性抽搐、神经抑制而死亡。存活大鼠在14天观察期内体重增长缓慢,余未见明显异常。柴胡总皂苷醇洗脱精制样品的大鼠急性毒性比柴胡总皂苷粗提取物的急性毒性大,但比小鼠急性毒性小。结论柴胡总皂苷是柴胡产生毒性的主要物质基础,其产生毒性的部位及毒性机制有待于深入研究。

Experimental Study on Rat's Acute Toxicity of Refined Products of Bupleurum Saikosaponin by Alcohol Elution

Objective To discuss the effect of refined products of Bupleurum saikosaponin by alcohol elution on rat＇s acute toxicity.Methods Make the study on rat＇s acute toxicity of the refined products of Bupleurum saikosaponin by alcohol elution in accordance with classical acute toxicity test methods.Results Caculated by total saikosaponin,the LD50 of the refined products of Bupleurum saikosaponin by alcohol elution is 591.95 mg·kg-1·d-1,and the 95% confidence limit is 490.96～713.7 mg·kg-1·d-1.The main acute toxicity symptoms and signs of dead rats are negligent action,then arising from unsteady gait,abdominal lying sleeping,rapid heart rate,shortness of breath,continuous convulsions,neurological inhibition,and eventually to death.The surviving rats＇ weight gainings are slow in 14d obervation period and there are no other obvious abnormalities.The acute toxicity of refined products of Bupleurum saikosaponin by alcohol elution is bigger than the total Bupleurum saikosaponin extracts,but it＇s smaller than the mice＇s.Conclusion The total saikosaponin is mainly toxical material basis of Bupleurum.The toxical location and mechanismare to be further studied.