Metabolism of D-[1-(13)C]fructose, D-[2-(13)C]fructose, and D-[6-(13)C]fructose in rat hepatocytes incubated in the presence of H(2)O or D(2)O |
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Authors: | Malaisse Willy J Ladrière Laurence Verbruggen Ingrid Willem Rudolph |
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Institution: | Laboratory of Experimental Medicine, Brussels Free University, Belgium. malaisse@med.ulb.ac.be |
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Abstract: | Isolated hepatocytes from fed rats were exposed for 120 min to D-1-(13)C]fructose, D-2-(13)C]fructose, or D-6-(13)C]fructose in the presence of H(2)O or D(2)O. The identification and quantification of (13)C-enriched metabolites (D-glucose, L-lactate) in the incubation medium and the measurement of their deuterated isotopomers indicated that the ketohexose was phosphorylated predominantly at the intervention of fructokinase and that the majority of the D-glyceraldehyde molecules generated from d-fructose 1-phosphate were further metabolized, e.g., after phosphorylation to D-glyceraldehyde 3-phosphate. It is proposed that the present procedure may help to further characterize the regulation of D-fructose metabolism in both hepatocytes and other cell types. |
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