胃癌细胞耐药相关蛋白质分子的差异展示

目的 寻找新的胃癌细胞耐药相关蛋白质分子，阐明肿瘤耐药的新机制。方法 以胃癌细胞SGC7901和长春新碱诱导的耐药胃癌细胞SGC7901／VCR为研究对象，用固相化pH梯度等电聚焦的二维聚丙烯酰胺凝胶电泳技术展示并比较两种细胞表达的所有蛋白质，凝胶银染法显示耐药与非耐药细胞差异表达的蛋白质分子。结果 在两张银染的凝胶蛋白质图谱上，均有680个可辨识的蛋白质点，绝大多数蛋白点在位置、形状和密度上是一致的。在耐药细胞蛋白质二维电泳图谱中，发现有30个明显差异的蛋白点，并初步确定了其等电点和分子量。其中3个蛋白点表达量很高，但在非耐药细胞中未出现；6个蛋白点丰度明显上调；19个蛋白点丰度明显下调；2个蛋白点在耐药细胞中未出现，但在非耐药细胞中高表达。结论 胃癌耐药细胞中差异表达的蛋白质分子可能与其长春新碱耐药机制相关。

Differntial display of vincristine-resistant proteins in gastric cancer cell line SC C7901

Objective To find out new multidrug resistant proteins in gastric cancer cells SGC7901 and to explain the new multidrug resistant mechanism of gastric cancer cells. Methods Two dimensional gel electrophoresis was used with immobilized pH gradients(IPG) to compare the differential expression of multidrug resistant proteins in gastric cancer cells SGC7901 and Vincristine resistant SGC7901 cells(SGC7901/VCR) induced by vincristine sulfate. 2 D gels were used to silver stain the protein. Results Approximately, 680 protein spots were identified in each of the 2 D gel patterns by silver stain. With most of them showing no difference in composition, shape or density, twenty five proteins were found to differ in quantity (6 higher in SGC7901/VCR cells; 19 higher in 7901 cells). Five proteins were seen to be unique in one region or the others (3 in SGC7901/VCR cells, 2 in 7901 cells). The coordinate position of 30 protein spots in the 2 D gels were listed. Conclusion The results suggest that these differential proteins be related to the vincristine resistant mechanism in human gastric cancer cell line SGC7901/VCR.