流式微球分析术检测输卵管炎性不孕症患者免疫指标的临床意义

目的 探讨流式微球分析术(CBA)检测输卵管炎性不孕症患者炎性细胞因子的临床意义.方法 用CBA法检测并分析40例输卵管炎性不孕患者和40名健康妇女血液中T淋巴细胞亚群、炎性细胞因子谱,并比较分析其中30例患者血清与腹腔积液炎性细胞因子谱的差异.结果 输卵管炎性不孕症患者T辅助(Th)细胞(CD3+CD4+)比健康组明显减少(t=2.1236,P<0.05),T抑制(Ts)细胞(CD3+CD8+)明显增多(f=2.4369,P<0.05),Th/T.比值显著性降低(t=2.8392,P<0.01).血清IL-8(t=4.9302,P<0.01)、IL-1B(t=5.5449,P<0.01)、IL-6(t=3.4975,P<0.01)、肿瘤坏死因子-α(TNF-α,t=5.6623,P<0.01)等炎性细胞因子,在输卵管炎性不孕症患者中都显著高于健康妇女;炎症抑制因子IL-10(t=5.3428,P<0.01)和IL-12(t=5.9847,P<0.01)也显著性升高.30例输卵管炎性不孕症患者血清与腹腔积液检测结果比较,显示除IL-10外,血液标本中IL-8(t=2.2113,P<0.05)、IL-1β(t=2.9756,P<0.01)、IL-6(t=2.3298,P<0.05)、TNF-α(t=3.2895,P<0.05)、IL-12(t=3.3281,P<0.05)均高于腹腔积液标本.结论 输卵管炎性不孕症患者存在炎性细胞因子网络的系统性紊乱;CBA能够高通量、高灵敏度地检测炎性细胞因子失调状况.

Cytometric bead assay about the inflammatory cytokines of salpingitic infertility

Objective To explore the abnormal inflammatory cytokine profile of salpingitic infertility by means of cytometric bead assay(CBA). Methods To assay representative inflammatory cytokines of 40 patients of salpingitic infertility(PSI) and 40 healthy control by CBA, and compared 30 samples of serum with peritoneal fluid collected from identical subject. Results Experiment data displayed that in PSI the Th lymphocytes were decreased significantly (t =2. 1236,P <0. 05), Ts lymphocytes increased markedly, and the ratio of Th/Ts markedly decreased ( t = 2. 8392, P < 0. 01 ), compared with those of healthy control.(Pre-)inflammatory eytokines (IL-8, IL-1β, IL-6 and TNF-α) were all significantly higher than those of healthy controls ( t = 4. 9302,5. 5449,3. 4975,5. 6623, respectively, all P < 0. 01 ) ; while anti-inflammatory cytokines ( IL-10, IL-12 ), also increased markedly ( t = 5. 3428,5.9847, respectively, all P < 0. 01 ).Beside IL-10, all other cytokine concentrations presented in serum were higher than that of peritoneal fluid;IL-8(t =2.2113,P <0.05),IL-llS(t =2.9756,P <0.01), IL-6(t =2.3298,P <0.05), TNF-α (t =3. 2895, P < 0. 05 ), IL-12 ( t = 3. 3281, P < 0. 05 ), implying more sensitive of serum samples. Conclusion PSI displayed extensive disorders of the inflammatory cytokine network, CBA can be used to probe the disharmony condition at high through-out and high sensitivity.