Variability among brain regions in the specificity of 6-hydroxydopamine (6-OHDA)-induced lesions |
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Authors: | D. L. Commins R. A. Shaughnessy K. J. Axt G. Vosmer L. S. Seiden |
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Affiliation: | (1) Present address: Department of Pharmacological and Physiological Sciences, The University of Chicago, Chicago, Illinois, USA |
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Abstract: | Summary 6-Hydroxydopamine (6-OHDA; 200 g, 150 g or 110 g) or vehicle was infused stereotaxically into the lateral ventricles of rats, usually following pretreatment with desmethylimipramine (DMI). Various brain regions were then assayed for dopamine (DA), serotonin (5-HT) and norepinephrine (NE). As expected, 6-OHDA depleted DA in all brain regions examined. Unexpectedly, however, the two highest doses of 6-OHDA significantly decreased 5-HT levels in the hippocampus and increased 5-HT levels in the striatum. In addition, despite pretreatment with doses of DMI commonly considered adequate to block 6-OHDA-induced depletion of NE, all doses of 6-OHDA tested significantly reduced NE levels in the hippocampus, hypothalamus and septum.We interpret our data as suggesting that some brain regions are susceptible to nonspecific toxic effects of 6-OHDA at doses commonly employed. Furthermore, these nonspecific effects may or may not occur, depending on seemingly minor variations in experimental technique. |
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Keywords: | 6-Hydroxydopamine desmethylimipramine dopamine serotonin norepinephrine reinforcements monoamines catecholamines neurotoxicity |
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