5种肽激素在黄鳝消化道内分泌细胞中的分布特点

目的 探索黄鳝消化道黏膜中5种内分泌细胞的定位及分布。方法 采用免疫细胞化学技术的链霉亲合素一生物素过氧化物酶复合物(SABC)法。结果 在黄鳝消化道黏膜中至少分布着5种免疫活性内泌细胞，它们是：胃泌素(Gastin，Gas)、生长抑素(Somatostatin，Som)、五羟色胺(5-Hydroxytryptamine，5-HT)、胰岛(Insulin，Ins)和神经丝蛋白一免疫活性内分泌细胞(Neurofilament—immunoreactlve cell，NF)。其中Gas和Sore细胞分布食道复层扁平上皮与杯状细胞之间：贲门处有少量Gas细胞分布，胃底上皮和胃腺中有大量的Sore细胞分布，Ins、NF和5-HT细胞则分别分布在幽门上皮和幽门腺管上。黄鳝的肠中则未见到上述任何一种免疫活性阳性应。另外，胰高血糖素在黄鳝消化道任何部位均未见到免疫活性阳性反应。以上各免疫活性内分泌细胞均为深色，形状不规则，胞突较短而粗，胞核呈空泡状，常夹于食道和胃上皮之间或分布于腺上皮之间，将胞突伸向胃腔腺腔。结论 作为最低等脊椎动物，黄鳝的消化道具有复杂的内分泌功能。

THE DISTRIBUTON CHARACTERISTIC OF FIVE PEPTIDE HORMONES IN THE ENDOCRINE CELLS IN DIGESTIVE TRACT OF MONOPTERUS ALBUS

Objective To investigate the localization and distribution of the five endocrine cells in the digestive tract mucosa of ricefield eel(Monopterus albus). Methods Using immunocytochemical technique of strept avidin-biotin-peroxidase complex(SABC) were used. Results At least 5 kinds of immunoreactive endocrine cells distributed in the digestive tract mucosa of M.albus. They were gastrin(Gas),somatostatin(Som),5-hydroxytryptamine(5-HT),insulin(Ins),and neurofilament (NF) immunoreactive endocrine(IRE) cells.Gas and Som-IRE cells distributed between stratified squamous epithelium and goblet cell in esophagus. A large number of Gas-IRE cells were found between gastric fundus epithelium and gastric glands, and only a few in the carcia. Ins, 5-HT and NF-IRE cells distributed in the epithelium pylorus and pyloric glandular tube respectively. No any immunoreactive positive reaction was found in the gut of M.albus.In addition, immunoreactive positive reaction of glucagons was not found in whole digestive tract.All immunoreactive endocrine cells were dark brown in color.Their morphology was irregular, cytoplasmic process was shorter and thicker, their nucleus showed an empty bubble.They distributed between esophageal epithelium and gastric epithelium or glandular epithelium, and cytoplasmic process extended to the gastric lumen and glandular cavity.Conclusion There is a complex endocrine function of the digestive system in ricefield eel (M.albus) at the lowest vertebrate.