| 聚酰胺柱层析-紫外可见分光光度法测定桑叶中总黄酮的含量 |
| |
| 引用本文： | 穆春旭,张建军,张振秋. 聚酰胺柱层析-紫外可见分光光度法测定桑叶中总黄酮的含量[J]. 黑龙江医药, 2012, 25(3): 342-343 |
| |
| 作者姓名： | 穆春旭 张建军 张振秋 |
| |
| 作者单位： | 1. 辽宁卫生职业技术学院
2. 辽宁中医药大学 |
| |
| 摘 要： | 目的:采用柱层析-紫外可见分光光度法测定6批桑叶中总黄酮的含量。方法:以芦丁为对照品,510nm处测定桑叶中总黄酮的吸收度。结果:线性范围为0.0090～0.0452mg/ml,r=0.9999,平均回收率为98.5%,RSD为1.3%。结论:该方法测定桑叶中总黄酮,方法简便,灵敏度和准确度高。
|
| 关 键 词： | 桑叶 总黄酮 紫外可见分光光度法 |
Determination of the Content of Total Flavonoid in Leaves of Morus alba L.by Spectrophotometry |
| |
| Affiliation: | Mu Chunxu1,et al 1. Liaoning College of Health Vacational technology |
| |
| Abstract: | Objective:Content of total flavoniod in leaves of Morus alba L.was determined by spectrophotometry.Methods:The detection was set at 510nm.Results:The method was linear with in the range of 0.0090~0.0452mg/ml,r =0.9999. The average recovery of assay was 98.5% and RSD=1.3% with reference to Rutin.Conclusion:The mothod is simple and convenient,high sensitivity and accuracy. It can be used for the determination of total flavonoid. |
| |
| Keywords: | Leaves of Morus alba Total Flavonoid spectrophotometry |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
