Serial indium-111-labelled IgG biodistribution in ratPneumocystis carinii pneumonia: a tool to monitor the course and severity of the infection |
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| Authors: | Roos M. Perenboom Wim J. G. Oyen Anita C. H. W. van Schijndel Pieter Beckers Frans H. M. Corstens Jos W. M. van der Meer |
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| Affiliation: | (1) Department of General Internal Medicine, University Hospital Nijmegen, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands;(2) Department of Nuclear Medicine, University Hospital Nijmegen, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands;(3) Department of Medical Microbiology, University Hospital Nijmegen, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands |
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| Abstract: | To study the effect of new therapeutic strategies, we developed an animal model to monitor the course and severity of experimentalPneumocystis carinii pneumonia (PCP) in rats.P. carinii density scores in Giemsa-stained impression smears were used to followP. carinii load. Indium-111 labelled IgG scintigraphy and biodistribution, histology of paraffin-embedded tissue sections, lung body weight (L/B wt) ratio and cell count and differentiation of broncho-alveolar lavage (BAL) fluid were used as parameters of host inflammatory response. Statistically significant differences in L/B wt ratio, number of neutrophils in BAL fluid,P. carinii density score, histological extent of inflammation and111In-IgG accumulation in the lung were seen between the rats sacrificed at various time points.111In-IgG accumulation in the lung correlated well with L/B wt ratio andP. carinii density score and correlated moderately with number of neutrophils in BAL fluid and with the histological extent of inflammation. |
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| Keywords: | Pneumocystis carinii pneumonia Indium-111 Biodistribution IgG scan |
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