2例TTV感染者中TTV DNA的基因变异分析

目的探讨TTV的基因变异情况。方法PCR扩增BD与CSH血清中的TTV DNA后进行分子克隆,每例挑选10个不同TTV DNA克隆进行测序。不同克隆之间及其与G1 a亚型TA278之间进行序列比较并进行进化树分析。结果BD存在2种不同的TTV病毒株,都为G1 a亚型。CSH中存在7种不同的TTV病毒株,分别属于G1 a和G1b亚型。结论CSH中TTV的基因变异比BD中的复杂得多。

Gene quasispecies analysis of transfusion transmitted virus DNA in two patients with transfusion transmitted virus infection

Objective To investigate gene variation and the relationship between gene variation and pathogenicity of transfusion transmitted virus(TTV).Methods The TTV DNA in the serum sample from a blood donor(BD) and a chronic non-A-G severe hepatitis(CSH) patient with TTV infection was amplified by using PCR.The purified PCR product was cloned and 10 clones from each case were sequenced.The sequences were compared among different clones and analyzed by Phylogentic tree.Results There were two different TTV strains in the BD and seven different TTV strains in the chronic non-A-G severe hepatitis patient.The TTV clones in the BD were of G1a subtype and those of the CSH were of G1a and G1b subtype.Conclusion Gene variant of TTV was much more complicated in the CSH patients than that in the BD ones.