降压益肾配方颗粒对SHR早期肾脏损害保护作用观察

目的：通过检测自发性高血压大鼠（SHR）肾脏胶原蛋白量及电镜观察肾动脉内皮的结构，观察降压益肾配方颗粒对高血压病早期肾损害的保护作用。方法：将SHR随机分为降压益肾配方颗粒大、小剂量组，尼群地平对照组，生理盐水组。分别测定治疗前、后各点的血压水平；治疗8周后收集24h尿液，放免法测定尿MAlb、尿β2-MG；处死SHR，取肾脏及肾动脉作图像分析和电镜扫描。结果：降压益肾配方颗粒各组与尼群地平组比较，降压疗效无显著差异（P〉0．05）；治疗组尿MAlb、尿β2-MG的排泄优于对照组（P〈0．05）；治疗组能明显降低肾小球胶原蛋白含量（P〈0．05），逆转肾实质损害。结论：降压益肾配方颗粒具有确切的降压疗效，能改善尿β2-MG、尿MAlb、逆转肾动脉及肾实质的损害，具有明确的保护肾脏的功能。

Observation of Protective Effect of Antihypertensive Kidney-Replenishing Formula Granule on Early Kidney Damage in Spontaneous Hypertensive Rats

Objective：to observe the protective effect of antihypertensive kidney-replenishing formula granule on early kidney damage by detecting content of renal collagen protein and observing structure of endodermis of arteria renalis with electron microscope. Methods ~ the spontaneous hypertensive rats were randomly divided to high dosage granule group, low dosage granule group, Nitrendipine group, and sodium chloride group. Blood pressure were detected prior to and after treatment. After 8 weeks of treatment, urine at 24 hour point was collected. Malb.β2-MG were detected with radio-immunity method ,the rats were sacrificed to analyze kidney and arteria renalis by image analyzer and electron microscope. Results：there was no significant difference between antihypertensive effect of the granule groups and that of the Nitrendipine group （P 〉 0. 05） ; the treatment groups had better excretion of Malb. β2-MG than the control group （P 〈 0.05） ; The treatment group could significantly bring down the content of renal collagen protein by which kidney parenchyma damage could be reversed （P 〈 0. 05）. Condusion： antihypertensive kidney-replenishing formula granule has definite antihypertensive and kidney protection effect, as shown by promotion of excretion of Malb .β2-MG, and reversion of damage to renal parenchyma and arteria renalis.