星点设计-效应面法优化健脾颗粒的提取工艺

目的:以星点设计-效应面法优选健脾颗粒的提取工艺。方法:以加水量和提取时间为自变量,以橙皮苷转移率为因变量对自变量各水平进行多元线性回归和二项式拟合,用效应面法选择较佳工艺条件,并进行预测分析。结果:确定较优提取工艺为加11倍量水,提取时间80 min,提取2次,提取率实测值与预测值偏差为0.54%,二项式拟合复相关系数r=0.958 3。结论:采用星点设计-效应面法优选健脾颗粒的提取工艺,方法简便,预测性良好。

Optimization of the Extraction Process of Jianpi Granules by Central Composite Design-Response Surface Methodology

ABSTRACT Objective:To optimize the extraction process of Jianpi granules by central composite design-response surface methodology. Method:The independent variables were extraction time and water yield, and the dependent variable was extraction ratio of hesperidin. Linear or no-linear mathematic models were used to estimate the relationship between the independent and dependent variables. Response surface methodology was used to optimize the extraction process. Prediction was carried out through comparing the observed and predicted values.Result:The regression coefficient of binomial fitting complex model was as high as 0.958 3, the optimum extraction conditions were 80 min extraction, 11-fold water and twice extraction. The bias between the observed and predicted values was 0.54%.Conclusion: Central composite design response surface methodology is convenient and highly predictive for optimizing the extraction process of Jianpi granules.