变应性接触性皮炎模型鼠体内Th17细胞活性变化

目的:研究变应性接触性皮炎(allergic contact dermatitis,ACD)模型鼠外周血及皮损处Th17细胞活性变化情况及其意义。方法:利用2,4-二硝氟苯(DNFB)诱导构建ACD小鼠模型,分别于激发前及激发后的第3、6、9、12、24 h取小鼠耳组织及外周血,评估小鼠耳组织炎症情况,采用实时荧光定量PCR法检测小鼠皮损处IL-17及RORγt mRNA的表达水平,利用酶联免疫吸附试验检测血清中IL-17水平。结果:激发后24 h内小鼠耳组织肿胀程度逐渐加重,皮损中IL-17和RORγt mRNA表达量也逐渐升高,激发后各组与激发前组比较差异有统计学意义(均P<0.05);耳组织IL-17和RORγt mRNA表达量与其肿胀程度呈正相关;激发后小鼠血清中IL-17水平升高,在6小时达到最高,随后逐渐下降。结论:在ACD小鼠的急性炎症期,皮损及外周血中Th17细胞活性增强,并且局部皮损处的Th17细胞活性与炎症程度密切正相关。

Changes of Activity of Th17 Cells in a Mouse Model of Allergic Contact Dermatitis

Objective ：To study the changes and significance of Thl7 cells activity in a mouse model of allergic contact dermatitis （ACD）. Methods：The ACD mouse model was established with 2,4- dinitrofluorobenzene （DNFB）. Ear swelling response was observed at six time points： before elicitation, 3h,6h,9h,12h,24h after elicitation. Meanwhile RT-RCR was conducted to detect the mRNA of IL-17 and RORγt in ear tissues. The level of IL-17 in peripheral blood was de- tected by ELISA. Results： The degree of inflammation and the expression of both IL-17 and RORγt mRNA in ear tissues went up gradually after elicitation. They were significantly higher than that before elicitation （P 〈 0. 05 ）. The degree of inflammation was positively correlated with the expression of IL-17 and RORγt mRNA in ear tissues. The level of IL-17 in peripheral blood went up to the peak 6h after elicitation and then declined gradually. Conclusion：The results demonstrate the activity of Th17 cells increase in acute inflammation of the ACD mouse model. The degree of inflammation is significantly positively correlated to the activity of Th17 cells in ear tissues.