大气颗粒物对大鼠血液及内皮损伤作用

目的 观察大气可吸入颗粒物一次气管滴注染毒后,大鼠血液炎症和内皮损伤指标的改变,探讨颗粒物所致心血管效应的生物标志。方法 将32只体重为380~470 g的雄性Wistar大鼠随机分为4组,即空白对照组、颗粒物低、中、高剂量组(0,5,15和45 mg/kg);采用气管滴注染毒,24 h后处死动物,分类计数血细胞,测定血液炎性指标白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、超敏C-反应蛋白(hs-CRP)以及内皮损伤指标一氧化氮(NO)和血管性假血友病因子(vWf)水平。结果 各染毒组大鼠血液白细胞总数明显升高,高剂量组达(7.94±1.15)×10⁹/L、中性粒细胞百分比增加至(40.50±5.75)%;血清IL-6、hs-CRP水平与对照组比较明显升高(P<0.05);中、高剂量组TNF-α水平较对照组明显升高(P<0.05),各染毒组血清NO、vWf水平与对照组比较,差异无统计学意义。结论 可吸入颗粒物染毒后可引起大鼠系统炎症反应,但未见内皮损伤指标的改变。

Acute changes of blood and endothelial damages in rats intratracheally instilled with PM_(10)

Objective To observe the systemic inflammatory changes of blood, endothelial damages, and cardiovascular effects caused by PM_(10). Methods Thirty two male Wistar rats weighted 380 -470g were randomly divided into one control group and three PM_(10) exposed groups. PM_(10)were administered to the rats by intratracheal instillation at the doses of 5,15, 45mg/kg·bw,respectively. The rats were sacrificed 24 hours after the treatment. The number of blood cells and the levels of IL-6 ,TNF-α, hypersensitive C-reactive protein (hs-CRP), nitric oxide and Von Willebrand factor in the serum were deter-mined. Results The number of white blood cells,the percentage of neutrophils,the levels of IL-6,and hs-CRP in the serum increased significantly in all the PM_(10)treated rats. TNF-α level in the serum increased markedly in 15 and 45mg/kg PM_(10) treated rats. No significant change was found in the levels of nitric oxide and Von Willebrand factor in the serum of PM_(10) treated rats. Conclusion The study showed that PM_(10) can cause systemic inflammation. However, there is no significant change in the blood indices for endothelial injury.